Identification of Human Antigen-Specific CD4+ Cells with Peptide-MHC Multimer Technologies.
Antigen-specific CD4 T cells
Epitopes
MHC class II multimers
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
24
6
2021
Statut:
ppublish
Résumé
Peptide-major histocompatibility complex class II (pMHCII) multimers have emerged as a convenient and powerful tool for characterization of CD4 T cell immune responses in a large variety of human diseases. Peptide-MHCII multimers can rapidly identify peptide antigens recognized by CD4 T cells via high-throughput peptide screening procedures. The specificity and phenotype of antigen-specific CD4 T cells can be effectively visualized by pMHCII multimers from unmanipulated immune cell populations. Functional attributes of antigen-specific CD4 T cells can also be defined with the multimer technology in combination with immune functional assays such as intracellular cytokine staining (ICS).
Identifiants
pubmed: 33928551
doi: 10.1007/978-1-0716-1311-5_13
doi:
Substances chimiques
Cytokines
0
Epitopes
0
Histocompatibility Antigens Class II
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
153-163Subventions
Organisme : NIDDK NIH HHS
ID : DP3 DK106909
Pays : United States
Références
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