M. fortuitum-induced CNS-pathology: Deciphering the role of canonical Wnt signaling, blood brain barrier components and cytokines.


Journal

Developmental and comparative immunology
ISSN: 1879-0089
Titre abrégé: Dev Comp Immunol
Pays: United States
ID NLM: 7708205

Informations de publication

Date de publication:
09 2021
Historique:
received: 10 12 2020
revised: 24 04 2021
accepted: 24 04 2021
pubmed: 3 5 2021
medline: 3 3 2022
entrez: 2 5 2021
Statut: ppublish

Résumé

Molecular underpinning of mycobacteria-induced CNS-pathology is not well understood. In the present study, zebrafish were infected with Mycobacterium fortuitum and the prognosis of CNS-pathogenesis studied. We observed M. fortuitum triggers extensive brain-pathology. Evans blue extravasation demonstrated compromised blood-brain barrier (BBB) integrity. Further, decreased expression in tight-junction (TJ) and adherens junction complex (AJC) genes were noted in infected brain. Wnt-signaling has emerged as a major player in host-mycobacterial immunity but its involvement/role in brain-infection is not well studied. Sustained expression of wnt2, wnt3a, fzd5, lrp5/6 and β-catenin, with concordant decline in degradation complex components axin, gsk3β and β-catenin regulator capn2a were observed. The surge in ifng1 and tnfa expression preceding il10 and il4 suggested cytokine-interplay critical in M. fortuitum-induced brain-pathology. Therefore, we suggest adult zebrafish as a viable model for studying CNS-pathology and using the same, conclude that M. fortuitum infection is associated with repressed TJ-AJC gene expression and compromised BBB permeability. Our results implicate Wnt/β-catenin pathway in M. fortuitum-induced CNS-pathology wherein Th1-type signals facilitate bacterial clearance and Th2-type signals prevent the disease sequel.

Identifiants

pubmed: 33933535
pii: S0145-305X(21)00119-1
doi: 10.1016/j.dci.2021.104111
pii:
doi:

Substances chimiques

Axin Protein 0
Cytokines 0
Low Density Lipoprotein Receptor-Related Protein-5 0
Low Density Lipoprotein Receptor-Related Protein-6 0
Receptors, Cell Surface 0
Tumor Necrosis Factor-alpha 0
Wnt Proteins 0
Wnt3A Protein 0
Wnt3a protein, zebrafish 0
Zebrafish Proteins 0
axin2 protein, zebrafish 0
beta Catenin 0
ctnnb1 protein, zebrafish 0
fzd5 protein, zebrafish 0
wnt2ba protein, zebrafish 0
Interleukin-10 130068-27-8
Interleukin-4 207137-56-2
Interferon-gamma 82115-62-6
Glycogen Synthase Kinase 3 beta EC 2.7.11.1
Calpain EC 3.4.22.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

104111

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Shagun Sharma (S)

Immunobiology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007, India.

Manmohan Kumar (M)

Immunobiology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007, India.

Jai Kumar (J)

Immunobiology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007, India.

Nidhi Srivastava (N)

Immunobiology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007, India; Department of Zoology, School of Basic and Applied Sciences, Maharaja Agrasen University, Solan, Himachal Pradesh, 174103, India.

Md Arafat Hussain (MA)

Immunobiology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007, India.

Asha Shelly (A)

Immunobiology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007, India.

Shibnath Mazumder (S)

Immunobiology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007, India; Faculty of Life Sciences and Biotechnology, South Asian University, Delhi, 110021, India. Electronic address: shibnath@sau.int.

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Classifications MeSH