High-efficiency transformation of Streptococcus thermophilus using electroporation.
Streptococcus thermophilus
competence genes expression
electroporation
transformation efficiency
Journal
Journal of the science of food and agriculture
ISSN: 1097-0010
Titre abrégé: J Sci Food Agric
Pays: England
ID NLM: 0376334
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
revised:
20
04
2021
received:
14
02
2021
accepted:
03
05
2021
pubmed:
4
5
2021
medline:
16
11
2021
entrez:
3
5
2021
Statut:
ppublish
Résumé
Streptococcus thermophilus, one of the most important lactic acid bacteria, is widely used in food fermentation, which is beneficial to improve food quality. However, the current genetic transformation systems are inefficient for S. thermophilus S-3, which hinders its further study. We developed three electroporation transformation methods for S. thermophilus S-3, and optimized various parameters to enhance the transformation efficiency up to 1.3 × 10 This is the first report of competence gene expression for enhancing transformability in S. thermophilus, which exerts a positive effect on the development of desirable characteristics strains. © 2021 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Streptococcus thermophilus, one of the most important lactic acid bacteria, is widely used in food fermentation, which is beneficial to improve food quality. However, the current genetic transformation systems are inefficient for S. thermophilus S-3, which hinders its further study.
RESULTS
RESULTS
We developed three electroporation transformation methods for S. thermophilus S-3, and optimized various parameters to enhance the transformation efficiency up to 1.3 × 10
CONCLUSION
CONCLUSIONS
This is the first report of competence gene expression for enhancing transformability in S. thermophilus, which exerts a positive effect on the development of desirable characteristics strains. © 2021 Society of Chemical Industry.
Substances chimiques
Bacterial Proteins
0
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6578-6585Subventions
Organisme : Shanghai Agriculture Applied Technology Development Program
ID : 2019-02-08-00-07-F01152
Organisme : National Natural Science Foundation of China
ID : 31871776
Organisme : National Natural Science Foundation of China
ID : 31972101
Organisme : National Science Fund for Distinguished Young Scholars
ID : 32025029
Organisme : Natural Science Foundation of Shanghai
ID : 18ZR1426800
Organisme : Shanghai Engineering Research Center of Food Microbiology
ID : 19DZ2281100
Informations de copyright
© 2021 Society of Chemical Industry.
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