Ribonuclease Protection: Mapping RNA with Ribonuclease and Radiolabeled RNA Probes.

Nuclease Protection Assays / methods Nucleic Acid Hybridization RNA / analysis RNA Probes RNA, Messenger / analysis Ribonuclease T1 / metabolism Ribonuclease, Pancreatic / metabolism Ribonucleases

Journal

Cold Spring Harbor protocols

ISSN: 1559-6095

Titre abrégé: Cold Spring Harb Protoc

Pays: United States

ID NLM: 101524530

Informations de publication

Date de publication:
03 05 2021

Historique:

entrez: 4 5 2021

pubmed: 5 5 2021

medline: 15 12 2021

Statut: epublish

Résumé

In this protocol a randomly labeled single-stranded RNA probe is prepared and then hybridized to a population of mRNA molecules. The RNAs are digested with a mixture of RNase A and RNase T1. The hybrid molecules, which are resistant to the RNases, are separated and analyzed using gel electrophoresis and radiography.

Identifiants

DOI: 10.1101/pdb.prot101832 PMID: 33941667

pubmed: 33941667

pii: 2021/5/pdb.prot101832

doi: 10.1101/pdb.prot101832

doi:

Substances chimiques

RNA Probes 0

RNA, Messenger 0

RNA 63231-63-0

Ribonucleases EC 3.1.-

Ribonuclease T1 EC 3.1.27.3

Ribonuclease, Pancreatic EC 3.1.27.5

Types de publication

Journal Article

Langues

eng

Ribonuclease Protection: Mapping RNA with Ribonuclease and Radiolabeled RNA Probes.

Journal

Informations de publication

Résumé

Identifiants

Substances chimiques

Types de publication

Langues

Sous-ensembles de citation

Informations de copyright

Auteurs

Michael R Green (MR)

Joseph Sambrook (J)

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Classifications MeSH