Single-Molecule FRET of Membrane Transport Proteins.
FRET
membrane proteins
molecular dynamics
single-molecule studies
transmembrane transporter
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
02 09 2021
02 09 2021
Historique:
revised:
03
05
2021
received:
08
03
2021
pubmed:
5
5
2021
medline:
11
2
2022
entrez:
4
5
2021
Statut:
ppublish
Résumé
Uncovering the structure and function of biomolecules is a fundamental goal in structural biology. Membrane-embedded transport proteins are ubiquitous in all kingdoms of life. Despite structural flexibility, their mechanisms are typically studied by ensemble biochemical methods or by static high-resolution structures, which complicate a detailed understanding of their dynamics. Here, we review the recent progress of single molecule Förster Resonance Energy Transfer (smFRET) in determining mechanisms and timescales of substrate transport across membranes. These studies do not only demonstrate the versatility and suitability of state-of-the-art smFRET tools for studying membrane transport proteins but they also highlight the importance of membrane mimicking environments in preserving the function of these proteins. The current achievements advance our understanding of transport mechanisms and have the potential to facilitate future progress in drug design.
Identifiants
pubmed: 33945656
doi: 10.1002/cbic.202100106
pmc: PMC8453700
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
2657-2671Subventions
Organisme : German-Israeli Foundation
ID : G-1288-207.9/2015
Organisme : BMBF
ID : 05K18YEA
Informations de copyright
© 2021 The Authors. Published by Wiley-VCH GmbH.
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