Automated Intracellular Pharmacological Electrophysiology for Ligand-Gated Ionotropic Receptor and Pharmacology Screening.

Animals CHO Cells Cricetulus Drug Evaluation, Preclinical HEK293 Cells Humans Mice Neurons / cytology Patch-Clamp Techniques / instrumentation Primary Cell Culture Rats Receptors, GABA-A / metabolism Robotics

Journal

Molecular pharmacology
ISSN: 1521-0111
Titre abrégé: Mol Pharmacol
Pays: United States
ID NLM: 0035623

Informations de publication

Date de publication:
07 2021
Historique:
received: 28 10 2020
accepted: 08 04 2021
pubmed: 8 5 2021
medline: 25 9 2021
entrez: 7 5 2021
Statut: ppublish

Résumé

Communication between neuronal cells, which is central to brain function, is performed by several classes of ligand-gated ionotropic receptors. The gold-standard technique for measuring rapid receptor response to agonist is manual patch-clamp electrophysiology, capable of the highest temporal resolution of any current electrophysiology technique. We report an automated high-precision patch-clamp system that substantially improves the throughput of these time-consuming pharmacological experiments. The patcherBot

Identifiants

DOI: 10.1124/molpharm.120.000195 PMID: 33958481 PMC: PMC8274318
pubmed: 33958481
pii: molpharm.120.000195
doi: 10.1124/molpharm.120.000195
pmc: PMC8274318
doi:

Substances chimiques

Receptors, GABA-A 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

73-82

Subventions

Organisme : NIDA NIH HHS
ID : R01 DA029639
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH117315
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS102727
Pays : United States
Organisme : NIDA NIH HHS
ID : T90 DA032466
Pays : United States
Organisme : NIMH NIH HHS
ID : U01 MH106027
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY023173
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS089719
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS111280
Pays : United States
Organisme : NINDS NIH HHS
ID : R35 NS111619
Pays : United States

Informations de copyright

Copyright © 2021 by The American Society for Pharmacology and Experimental Therapeutics.

Références

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Auteurs

Riley E Perszyk (RE)

George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.).

Mighten C Yip (MC)

George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.).

Ona L McConnell (OL)

George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.).

Eric T Wang (ET)

George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.).

Andrew Jenkins (A)

George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.) cforest@gatech.edu ajenki2@emory.edu strayne@emory.edu.

Stephen F Traynelis (SF)

George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.) cforest@gatech.edu ajenki2@emory.edu strayne@emory.edu.

Craig R Forest (CR)

George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.) cforest@gatech.edu ajenki2@emory.edu strayne@emory.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Automated Intracellular Pharmacological...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules CHO › Automated Intracellular Pharmacological...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Arvicolinae › Cricetinae › Cricetulus › Automated Intracellular Pharmacological...
questionsmedicales.fr › Techniques d'investigation › Évaluation préclinique de médicament › Automated Intracellular Pharmacological...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Automated Intracellular Pharmacological...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Automated Intracellular Pharmacological...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Automated Intracellular Pharmacological...
questionsmedicales.fr › Cellules › Neurones › Automated Intracellular Pharmacological...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Techniques de patch-clamp › Automated Intracellular Pharmacological...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Culture de cellules primaires › Automated Intracellular Pharmacological...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Automated Intracellular Pharmacological...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs aux neuromédiateurs › Récepteurs aux acides aminés › Récepteurs GABA › Récepteurs GABA-A › Automated Intracellular Pharmacological...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Algorithmes › Intelligence artificielle › Robotique › Automated Intracellular Pharmacological...