Mass spectrometry informs the structure and dynamics of membrane proteins involved in lipid and drug transport.
Journal
Current opinion in structural biology
ISSN: 1879-033X
Titre abrégé: Curr Opin Struct Biol
Pays: England
ID NLM: 9107784
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
08
03
2021
accepted:
30
03
2021
pubmed:
9
5
2021
medline:
4
11
2021
entrez:
8
5
2021
Statut:
ppublish
Résumé
Membrane proteins are important macromolecules that play crucial roles in many cellular and physiological processes. Over the past two decades, the use of mass spectrometry as a biophysical tool to characterise membrane proteins has grown steadily. By capturing these dynamic complexes in the gas phase, many unknown small molecule interactions have been revealed. One particular application of this research has been the focus on antibiotic resistance with considerable efforts being made to understand underlying mechanisms. Here we review recent advances in the application of mass spectrometry that have yielded both structural and dynamic information on the interactions of antibiotics with proteins involved in bacterial cell envelope biogenesis and drug efflux.
Identifiants
pubmed: 33964676
pii: S0959-440X(21)00052-X
doi: 10.1016/j.sbi.2021.03.014
pii:
doi:
Substances chimiques
Lipids
0
Membrane Proteins
0
Pharmaceutical Preparations
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
53-60Subventions
Organisme : Medical Research Council
ID : MR/N020413/1
Pays : United Kingdom
Informations de copyright
Copyright © 2021 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest statement Nothing declared.