Generation and analysis of Prss28 and Prss29 deficient mice using CRISPR-Cas9 genome-editing.
Prss28
Prss29
gland
uterus
Journal
Molecular reproduction and development
ISSN: 1098-2795
Titre abrégé: Mol Reprod Dev
Pays: United States
ID NLM: 8903333
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
revised:
22
04
2021
received:
16
03
2021
accepted:
23
04
2021
pubmed:
12
5
2021
medline:
27
1
2022
entrez:
11
5
2021
Statut:
ppublish
Résumé
Glands of the uterus are essential for the establishment of pregnancy in mice and their products regulate embryo implantation and stromal cell decidualization critical for pregnancy establishment. Forkhead box A2 (FOXA2) is expressed specifically in the glands and a critical regulator of their differentiation, development and function. Progesterone and FOXA2 regulate members of a serine proteinase gene family (Prss28 and Prss29). Here, CRISPR-Cas9 genome-editing was used to create mice with a heterozygous or homozygous deletion of Prss28 or/and Prss29 to determine their biological roles in uterine function. Female mice lacking Prss28 and Prss29 or both developed normally and were fertile without alterations in uterine histoarchitecture, uterine gland number, or and gene expression. Thus, Prss28 and Prss29 are dispensable for female fertility and do not impact endometrial gland development or uterine function mice.
Identifiants
pubmed: 33973295
doi: 10.1002/mrd.23473
pmc: PMC8530251
mid: NIHMS1704080
doi:
Substances chimiques
Prss28 protein, mouse
EC 3.4.21.-
Prss29 protein, mouse
EC 3.4.21.-
Serine Endopeptidases
EC 3.4.21.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
482-489Subventions
Organisme : NICHD NIH HHS
ID : R01 HD096266
Pays : United States
Informations de copyright
© 2021 Wiley Periodicals LLC.
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