A hybrid aggregate FRET probe from the mixed assembly of cyanine dyes for highly specific monitoring of mitochondria autophagy.

Autophagosomes / metabolism Fluorescence Resonance Energy Transfer Lysosomes / metabolism Mitochondria Mitophagy
Cyanine dye FRET imaging Hybrid aggregate Mitochondria autophagy Mixed assembly

Journal

Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534

Informations de publication

Date de publication:
22 Jun 2021
Historique:
received: 17 01 2021
revised: 27 03 2021
accepted: 19 04 2021
entrez: 12 5 2021
pubmed: 13 5 2021
medline: 20 5 2021
Statut: ppublish

Résumé

Mitochondria autophagy, also known as mitophagy, is a process in which mitochondria are wrapped by autophagosomes and fused with lysosomes for degradation. This process is essential for mitochondrial quality control. Here, we developed a hybrid aggregate FRET probe through mixed assembly of two cyanine dyes FMOTY and AMTC. In live cells, FMOTY and AMTC exist independently in lysosomes and mitochondria and will not produce interfering FRET background signals. The FRET signal is only generated when mitochondria is transported to lysosomes during mitophagy. This allows the hybridized aggregate to be used as a highly specific probe for monitoring mitophagy.

Identifiants

DOI: 10.1016/j.aca.2021.338561 PMID: 33975703
pubmed: 33975703
pii: S0003-2670(21)00387-1
doi: 10.1016/j.aca.2021.338561
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

338561

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Xiaomeng Guo (X)

Beijing National Laboratory for Molecular Sciences (BNLMS), State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.

Qian Li (Q)

Center for Physicochemical Analysis and Measurement, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, PR China.

Junfeng Xiang (J)

Center for Physicochemical Analysis and Measurement, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, PR China.

Meirong Liu (M)

Center for Physicochemical Analysis and Measurement, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, PR China.

Aijiao Guan (A)

Beijing National Laboratory for Molecular Sciences (BNLMS), State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, PR China.

Yalin Tang (Y)

Beijing National Laboratory for Molecular Sciences (BNLMS), State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.

Hongxia Sun (H)

Beijing National Laboratory for Molecular Sciences (BNLMS), State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, PR China. Electronic address: hongxsun@iccas.ac.cn.

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Classifications MeSH

questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Phagosomes › Autophagosomes › A hybrid aggregate FRET probe from the mixed...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Transfert d'énergie par résonance de fluorescence › A hybrid aggregate FRET probe from the mixed...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Lysosomes › A hybrid aggregate FRET probe from the mixed...
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Mitochondries › A hybrid aggregate FRET probe from the mixed...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Renouvellement des mitochondries › Dégradation des mitochondries › A hybrid aggregate FRET probe from the mixed...