Characteristics of Electrospray-Ionization Detection of Synthetic Di- to Penta-Oligopeptides by Amine Derivatizations.

ESI-MS Oligopeptide chemical derivatization molecular surface area

Journal

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
ISSN: 1348-2246
Titre abrégé: Anal Sci
Pays: Switzerland
ID NLM: 8511078

Informations de publication

Date de publication:
10 Nov 2021
Historique:
pubmed: 18 5 2021
medline: 16 11 2021
entrez: 17 5 2021
Statut: ppublish

Résumé

Chemical derivatizations have been extensively developed for highly sensitive detection of bioactive small peptides; however, their advantages from the viewpoint of longer oligopeptides remain unverified. In this study, electrospray-ionization (ESI)-mass spectrometric (MS) detection of synthetic di- to pentapeptides consisting of glycine and sarcosine were characterized by four amine derivatization methods. It was concluded that the ESI-MS detection of di- to pentapeptides was characterized by the molecular surface area of derivatized peptide moieties with an optimal value of 250 - 300 Å

Identifiants

pubmed: 33994415
doi: 10.2116/analsci.21N016
pii: 10.2116/analsci.21N016
doi:

Substances chimiques

Amines 0
Oligopeptides 0
Peptides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1629-1632

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Auteurs

Weilin Shen (W)

Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan.

Kiyomi Ono (K)

Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan.

Mitsuru Tanaka (M)

Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan.

Toshiro Matsui (T)

Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan. tmatsui@agr.kyushu-u.ac.jp.

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