Molecular mechanism of Mad1 kinetochore targeting by phosphorylated Bub1.
Bub1
Cell cycle
Mad1
Mitotic checkpoint complex
Spindle assembly checkpoint
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
05 07 2021
05 07 2021
Historique:
revised:
02
04
2021
received:
09
12
2020
accepted:
13
04
2021
pubmed:
21
5
2021
medline:
12
8
2021
entrez:
20
5
2021
Statut:
ppublish
Résumé
During metaphase, in response to improper kinetochore-microtubule attachments, the spindle assembly checkpoint (SAC) activates the mitotic checkpoint complex (MCC), an inhibitor of the anaphase-promoting complex/cyclosome (APC/C). This process is orchestrated by the kinase Mps1, which initiates the assembly of the MCC onto kinetochores through a sequential phosphorylation-dependent signalling cascade. The Mad1-Mad2 complex, which is required to catalyse MCC formation, is targeted to kinetochores through a direct interaction with the phosphorylated conserved domain 1 (CD1) of Bub1. Here, we present the crystal structure of the C-terminal domain of Mad1 (Mad1
Identifiants
pubmed: 34013668
doi: 10.15252/embr.202052242
pmc: PMC8391104
doi:
Substances chimiques
Cell Cycle Proteins
0
Mad2 Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e52242Subventions
Organisme : Medical Research Council
ID : MC_U105184326
Pays : United Kingdom
Organisme : MRF
ID : MRF_MRF-110-057-S-RADA-C0940
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UP_1201/6
Pays : United Kingdom
Organisme : Cancer Research UK
ID : C576/A14109
Pays : United Kingdom
Informations de copyright
© 2021 MRC Laboratory of Molecular Biology. Published under the terms of the CC BY 4.0 license.
Références
Protein Sci. 2018 Jan;27(1):293-315
pubmed: 29067766
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Adv Biophys. 1976;:1-63
pubmed: 797240
Nat Rev Mol Cell Biol. 2013 Jan;14(1):25-37
pubmed: 23258294
Nat Cell Biol. 2012 Jun 03;14(7):746-52
pubmed: 22660415
Mol Cell. 2016 Aug 18;63(4):593-607
pubmed: 27522463
Methods Cell Biol. 2008;84:143-79
pubmed: 17964931
Elife. 2017 Jan 10;6:
pubmed: 28072388
Q Rev Biophys. 2000 May;33(2):161-97
pubmed: 11131563
Elife. 2013 Sep 24;2:e01030
pubmed: 24066227
Mol Cell. 2016 Dec 15;64(6):1144-1153
pubmed: 27939943
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
J Biol Chem. 2015 Jan 23;290(4):2431-43
pubmed: 25505175
Curr Biol. 2004 Jun 8;14(11):953-64
pubmed: 15182668
Curr Biol. 2000 Jun 1;10(11):675-8
pubmed: 10837255
Dev Cell. 2015 Dec 7;35(5):600-613
pubmed: 26651294
Bioinformatics. 2015 Apr 15;31(8):1325-7
pubmed: 25505092
EMBO Rep. 2021 Jul 5;22(7):e52242
pubmed: 34013668
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82
pubmed: 16369096
Curr Biol. 2012 May 22;22(10):900-6
pubmed: 22521787
Mol Cell. 2008 Aug 8;31(3):438-48
pubmed: 18691976
J Biomol NMR. 2014 Feb;58(2):129-39
pubmed: 24435565
Mol Cell. 2002 Jan;9(1):59-71
pubmed: 11804586
J Biomol NMR. 2004 Sep;30(1):25-35
pubmed: 15452432
Drug Discov Today. 2016 May;21(5):819-25
pubmed: 27032894
EMBO J. 2002 May 15;21(10):2496-506
pubmed: 12006501
Mol Cell Oncol. 2015 Jan 30;2(1):e970484
pubmed: 27308407
Nat Commun. 2015 Sep 24;6:8364
pubmed: 26399325
Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6549-54
pubmed: 22493223
Cells. 2019 Mar 23;8(3):
pubmed: 30909555
Mol Biol Cell. 2016 Nov 7;27(22):3405-3417
pubmed: 27170178
Mol Cell. 2017 Nov 16;68(4):715-730.e5
pubmed: 29129638
Curr Biol. 2004 Jun 8;14(11):942-52
pubmed: 15182667
Nat Cell Biol. 1999 Jun;1(2):82-7
pubmed: 10559878
Nature. 2017 Feb 23;542(7642):498-502
pubmed: 28102834
J Appl Crystallogr. 2011 Dec 1;44(Pt 6):1285-1287
pubmed: 22477786
Dev Cell. 2009 Jan;16(1):105-17
pubmed: 19154722
Biochemistry. 1996 Sep 3;35(35):11268-77
pubmed: 8784180
Mol Cell. 2015 Mar 5;57(5):824-835
pubmed: 25661489
Trends Cell Biol. 2015 Jan;25(1):21-8
pubmed: 25220181
J Biol Chem. 1982 Mar 25;257(6):2848-55
pubmed: 6277916
Nature. 2016 Aug 25;536(7617):431-436
pubmed: 27509861
PLoS Biol. 2009 Jan 13;7(1):e10
pubmed: 19143472
J Biomol NMR. 2011 Jun;50(2):157-65
pubmed: 21604143
Nat Struct Mol Biol. 2004 Apr;11(4):338-45
pubmed: 15024386
J Biol Chem. 2018 Jan 12;293(2):484-496
pubmed: 29162720
Nucleic Acids Res. 2019 Jul 2;47(W1):W402-W407
pubmed: 31251384
Genes Dev. 1996 Dec 15;10(24):3081-93
pubmed: 8985178
Science. 2009 Mar 13;323(5920):1477-81
pubmed: 19286556
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Nature. 1978 Jun 8;273(5662):443-6
pubmed: 661956
EMBO Rep. 2014 Mar;15(3):291-8
pubmed: 24477934
Nat Rev Mol Cell Biol. 2014 Nov;15(11):736-47
pubmed: 25303117
Curr Biol. 2012 May 22;22(10):891-9
pubmed: 22521786
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877
pubmed: 31588918
Protein Sci. 1995 Dec;4(12):2478-86
pubmed: 8580838
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
EMBO J. 2019 Apr 1;38(7):
pubmed: 30782962
Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11332-6
pubmed: 8248248
Protein Sci. 2004 Aug;13(8):2089-100
pubmed: 15273307
Methods Mol Biol. 2003;232:127-31
pubmed: 12840545
J Cell Biol. 2001 Sep 3;154(5):925-36
pubmed: 11535616
Curr Biol. 2015 Oct 19;25(20):R1002-18
pubmed: 26485365
Nat Commun. 2017 Jun 12;8:15822
pubmed: 28604727
Acta Crystallogr D Struct Biol. 2016 Mar;72(Pt 3):430-9
pubmed: 26960130
Cell. 2007 Nov 16;131(4):730-43
pubmed: 18022367
Genes Dev. 2014 Jan 15;28(2):140-52
pubmed: 24402315
Nature. 2012 Mar 21;484(7393):208-13
pubmed: 22437499
Front Cell Dev Biol. 2018 May 07;6:51
pubmed: 29868582
Curr Biol. 2005 Feb 8;15(3):214-25
pubmed: 15694304
Nature. 2015 Jan 29;517(7536):631-4
pubmed: 25383541
Mol Cell. 2014 Feb 20;53(4):591-605
pubmed: 24530301
Anal Biochem. 2003 Sep 1;320(1):104-24
pubmed: 12895474
Methods Enzymol. 2015;562:109-33
pubmed: 26412649
Nucleic Acids Res. 2007;35(6):1992-2002
pubmed: 17341463