Stepwise metabolic engineering of Candida tropicalis for efficient xylitol production from xylose mother liquor.
Candida tropicalis
Cofactor regeneration
Fermentation optimization
Xylitol
Xylose mother liquor
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
25 May 2021
25 May 2021
Historique:
received:
22
03
2021
accepted:
15
05
2021
entrez:
26
5
2021
pubmed:
27
5
2021
medline:
21
10
2021
Statut:
epublish
Résumé
Commercial xylose purification produces xylose mother liquor (XML) as a major byproduct, which has become an inexpensive and abundant carbon source. A portion of this XML has been used to produce low-value-added products such as caramel but the remainder often ends up as an organic pollutant. This has become an issue of industrial concern. In this study, a uracil-deficient Candida tropicalis strain was engineered to efficiently convert XML to the commercially useful product xylitol. The xylitol dehydrogenase gene was deleted to block the conversion of xylitol to xylulose. Then, an NADPH regeneration system was added through heterologous expression of the Yarrowia lipolytica genes encoding 6-phosphate-gluconic acid dehydrogenase and 6-phosphate-glucose dehydrogenase. After process optimization, the engineered strain, C. tropicalis XZX-B4ZG, produced 97.10 g L In conclusion, this study performed a combination of metabolic engineering and process optimizing in C. tropicalis to enhance xylitol production from XML. The use of C. tropicalis XZX-B4ZG, therefore, provided a convenient method to transform the industrial by-product XML into the useful material xylitol.
Sections du résumé
BACKGROUND
BACKGROUND
Commercial xylose purification produces xylose mother liquor (XML) as a major byproduct, which has become an inexpensive and abundant carbon source. A portion of this XML has been used to produce low-value-added products such as caramel but the remainder often ends up as an organic pollutant. This has become an issue of industrial concern. In this study, a uracil-deficient Candida tropicalis strain was engineered to efficiently convert XML to the commercially useful product xylitol.
RESULTS
RESULTS
The xylitol dehydrogenase gene was deleted to block the conversion of xylitol to xylulose. Then, an NADPH regeneration system was added through heterologous expression of the Yarrowia lipolytica genes encoding 6-phosphate-gluconic acid dehydrogenase and 6-phosphate-glucose dehydrogenase. After process optimization, the engineered strain, C. tropicalis XZX-B4ZG, produced 97.10 g L
CONCLUSIONS
CONCLUSIONS
In conclusion, this study performed a combination of metabolic engineering and process optimizing in C. tropicalis to enhance xylitol production from XML. The use of C. tropicalis XZX-B4ZG, therefore, provided a convenient method to transform the industrial by-product XML into the useful material xylitol.
Identifiants
pubmed: 34034730
doi: 10.1186/s12934-021-01596-1
pii: 10.1186/s12934-021-01596-1
pmc: PMC8147403
doi:
Substances chimiques
Xylose
A1TA934AKO
Glucose 1-Dehydrogenase
EC 1.1.1.47
Glucosephosphate Dehydrogenase
EC 1.1.1.49
D-Xylulose Reductase
EC 1.1.1.9
Xylitol
VCQ006KQ1E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105Subventions
Organisme : Natural Science Foundation of Jiangsu Province
ID : BK20171138
Organisme : the 111 Project
ID : 111-2-06
Organisme : Postgraduate Research and Practice Innovation Program of Jiangsu Province
ID : KYCX20-1807
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