Thin-Layer Chromatography.
Betaine lipid
Fatty acid
Glycerolipid
Glycolipid
Iodine
Nonpolar lipid
Phospholipid
Polar lipid
TLC
Wax ester
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
28
5
2021
pubmed:
29
5
2021
medline:
23
6
2021
Statut:
ppublish
Résumé
Lipid extracts from plants represent a mixture of polar membrane lipids and nonpolar lipids. The main constituents of the polar lipid fraction are glycerolipids, that is, galactolipids, sulfolipid, and phospholipids. In addition, betaine lipids are found in pteridophytes, bryophytes, and algae. Nonpolar lipids include the storage lipid triacylglycerol, wax esters, diacylglycerol and free fatty acids. The complex lipid mixtures from plant tissues can be separated by thin-layer chromatography (TLC) into different lipid classes. In most cases glass plates coated with a silica gel are used as stationary phase and an organic solvent as mobile phase. Different solvent systems are required to separate polar membrane lipids or nonpolar lipids by TLC. Depending on the complexity of the lipid mixture, lipids are separated using one- or two-dimensional TLC systems. Different dyes and reagents allow the visualization of all lipid classes, or the selective staining of glycolipids or phospholipids. Lipids can be isolated from the TLC plate for subsequent analysis, provided that nondestructive methods are used for visualization.
Identifiants
pubmed: 34047970
doi: 10.1007/978-1-0716-1362-7_3
doi:
Substances chimiques
Fatty Acids
0
Fatty Acids, Nonesterified
0
Galactolipids
0
Lipids
0
Membrane Lipids
0
Phospholipids
0
Solvents
0
sulfolipids
0
Glycerol
PDC6A3C0OX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
29-41Références
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