Lipid Analysis by Gas Chromatography and Gas Chromatography-Mass Spectrometry.
Alcohols
Double bond position
Fatty acids
Gas chromatography
Mass spectrometry
Methyl esters
Monoacylglycerols
Silylation
Thin-layer chromatography
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
28
5
2021
pubmed:
29
5
2021
medline:
23
6
2021
Statut:
ppublish
Résumé
Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) represent powerful tools for the quantitative and structural analysis of plant lipids. Here, we outline protocols for the isolation, separation, and derivatization of plant lipids for subsequent GC and GC-MS analysis. Plant lipids are extracted with organic solvents and separated according to their polarity by thin-layer chromatography or solid phase extraction. As most lipids are not volatile, the analytes are derivatized by transmethylation or trimethylsilylation to enable the transition of the molecules into the gas phase. After separation on the polymer matrix of the GC column, the analytes are detected by flame ionization or mass spectrometry. This chapter includes methods suitable for the analysis of lipid-bound or free fatty acids, long chain alcohols, and monoacylglycerols and for the determination of double bond positions in fatty acids.
Identifiants
pubmed: 34047971
doi: 10.1007/978-1-0716-1362-7_4
doi:
Substances chimiques
Fatty Acids
0
Fatty Acids, Nonesterified
0
Lipids
0
Membrane Lipids
0
Solvents
0
Glycerol
PDC6A3C0OX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
43-57Références
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