Neural Cell Adhesion Molecule Regulates Osteoblastic Differentiation Through Wnt/β-Catenin and PI3K-Akt Signaling Pathways in MC3T3-E1 Cells.
MC3T3-E1 cells
NCAM
PI3K-Akt signaling
Wnt/β-catenin signaling
osteoblast differentiation
Journal
Frontiers in endocrinology
ISSN: 1664-2392
Titre abrégé: Front Endocrinol (Lausanne)
Pays: Switzerland
ID NLM: 101555782
Informations de publication
Date de publication:
2021
2021
Historique:
received:
24
01
2021
accepted:
26
04
2021
entrez:
31
5
2021
pubmed:
1
6
2021
medline:
22
1
2022
Statut:
epublish
Résumé
Neural cell adhesion molecule (NCAM) is involved in cell multi-directional differentiation, but its role in osteoblast differentiation is still poorly understood. In the present study, we investigated whether and how NCAM regulates osteoblastic differentiation. We found that NCAM silencing inhibited osteoblast differentiation in pre-osteoblastic MC3T3-E1 cells. The function of NCAM was further confirmed in NCAM-deficient mesenchymal stem cells (MSCs), which also had a phenotype with reduced osteoblastic potential. Moreover, NCAM silencing induced decrease of Wnt/β-catenin and Akt activation. The Wnt inhibitor blocked osteoblast differentiation, and the Wnt activator recovered osteoblast differentiation in NCAM-silenced MC3T3-E1 cells. We lastly demonstrated that osteoblast differentiation of MC3T3-E1 cells was inhibited by the PI3K-Akt inhibitor. In conclusion, these results demonstrate that NCAM silencing inhibited osteoblastic differentiation through inactivation of Wnt/β-catenin and PI3K-Akt signaling pathways.
Identifiants
pubmed: 34054729
doi: 10.3389/fendo.2021.657953
pmc: PMC8150200
doi:
Substances chimiques
Neural Cell Adhesion Molecules
0
Wnt Proteins
0
beta Catenin
0
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
657953Informations de copyright
Copyright © 2021 Cheng, Feng, Gao, Jian, Liu, Wang, Xie, Wang, Feng and Yang.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Cell. 1997 May 30;89(5):765-71
pubmed: 9182764
J Biol Chem. 2005 Sep 30;280(39):33132-40
pubmed: 16043491
J Clin Invest. 2006 May;116(5):1202-9
pubmed: 16670761
J Biol Chem. 2002 Sep 6;277(36):33361-8
pubmed: 12084724
Expert Opin Pharmacother. 2016 Jun;17(8):1141-52
pubmed: 27055223
Exp Neurol. 2006 Mar;198(1):54-64
pubmed: 16336965
Arthritis Res Ther. 2014 Jul 03;16(4):R139
pubmed: 24994452
Int J Dev Neurosci. 2000 Apr-Jun;18(2-3):193-9
pubmed: 10715574
Exp Gerontol. 1998 Nov-Dec;33(7-8):853-64
pubmed: 9951628
Gene. 2006 May 10;372:62-70
pubmed: 16574347
J Cell Physiol. 2019 Aug;234(10):18075-18085
pubmed: 30847950
Cell. 1997 May 30;89(5):755-64
pubmed: 9182763
Ageing Res Rev. 2019 Dec;56:100964
pubmed: 31561015
Int J Mol Sci. 2019 Apr 04;20(7):
pubmed: 30987410
Nat Rev Immunol. 2007 Apr;7(4):292-304
pubmed: 17380158
J Rheumatol. 1999 Mar;26(3):622-6
pubmed: 10090173
J Biol Chem. 2011 Jul 22;286(29):26127-37
pubmed: 21628472
Ann N Y Acad Sci. 2009 Sep;1176:124-34
pubmed: 19796240
J Cell Sci. 2011 Aug 1;124(Pt 15):2552-60
pubmed: 21730021
Med Res Rev. 2018 Sep;38(6):2024-2057
pubmed: 29733451
Bone Res. 2013 Mar 29;1(1):27-71
pubmed: 26273492
Osteoarthritis Cartilage. 2007 Jan;15(1):69-77
pubmed: 16861013
Nat Rev Endocrinol. 2020 Feb;16(2):91-103
pubmed: 31792439
J Cell Biochem. 2018 Jan;119(1):748-757
pubmed: 28657691
J Bone Miner Res. 2010 Dec;25(12):2680-9
pubmed: 20593410
Endocrinol Metab Clin North Am. 2012 Sep;41(3):475-86
pubmed: 22877425
Nat Rev Rheumatol. 2016 Nov;12(11):632-644
pubmed: 27652499
Stem Cells. 2004;22(5):849-60
pubmed: 15342948
Med Sci Monit. 2019 Mar 29;25:2289-2295
pubmed: 30923307
Osteoporos Int. 1997;7 Suppl 3:S108-16
pubmed: 9536315
Cell. 2002 Jan 11;108(1):17-29
pubmed: 11792318
Biochem Biophys Res Commun. 2015 Sep 25;465(3):374-80
pubmed: 26255203
J Biomed Mater Res A. 2007 Dec 1;83(3):770-8
pubmed: 17559111
Osteoporos Int. 2009 Oct;20(10):1651-62
pubmed: 19415374
J Endocrinol. 2011 Nov;211(2):123-30
pubmed: 21673026
Bone Res. 2019 Aug 15;7:25
pubmed: 31646015
J Cell Biol. 2004 Jul 5;166(1):85-95
pubmed: 15226309
Stem Cells Transl Med. 2020 Feb;9(2):273-283
pubmed: 31742919
Structure. 2003 Jun;11(6):691-701
pubmed: 12791257
J Biol Chem. 2005 Dec 30;280(52):42971-7
pubmed: 16267048
Endocrinology. 2009 Jul;150(7):3067-75
pubmed: 19213846