Stress relief: emerging methods to mitigate dissociation-induced artefacts.
cell dissociation artefacts
dissociation-induced genes
early response genes
single-cell reference atlases
stress response
Journal
Trends in cell biology
ISSN: 1879-3088
Titre abrégé: Trends Cell Biol
Pays: England
ID NLM: 9200566
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
received:
12
03
2021
revised:
04
05
2021
accepted:
06
05
2021
pubmed:
3
6
2021
medline:
25
3
2022
entrez:
2
6
2021
Statut:
ppublish
Résumé
The rapid progress of single-cell RNA-sequencing (scRNA-seq) at large scales has led to what seemed impossible until recently: the generation of comprehensive transcriptional maps of nearly all cells in multicellular tissues. We pinpoint three key elements as being critical to the production of these maps: scalability, spatial information, and accuracy of the transcriptome of the individual cells. Here, we discuss the ramifications of traditional cell-isolation protocols when capturing the transcriptional signature of cells as they exist in their native tissue context, the methods that have been developed to avoid these distortions, and the biological processes that have unraveled on account of these upgraded methodological approaches.
Identifiants
pubmed: 34074577
pii: S0962-8924(21)00096-9
doi: 10.1016/j.tcb.2021.05.004
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
888-897Informations de copyright
Copyright © 2021 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.