Identification and characterization of microRNAs in the immature stage of the beneficial predatory bug Arma chinensis Fallou (Hemiptera: Pentatomidae).
Arma chinensis
development
microRNA
noncoding RNA
nymph
Journal
Archives of insect biochemistry and physiology
ISSN: 1520-6327
Titre abrégé: Arch Insect Biochem Physiol
Pays: United States
ID NLM: 8501752
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
revised:
29
04
2021
received:
07
01
2021
accepted:
16
05
2021
pubmed:
3
6
2021
medline:
6
7
2021
entrez:
2
6
2021
Statut:
ppublish
Résumé
MicroRNAs (miRNAs) are a type of small noncoding RNAs that regulate gene expression at the posttranscriptional level and can influence significant biological processes. Arma chinensis (Hemiptera: Pentatomidae) is a predaceous insect species that preys upon a wide variety of insect pests. It is important to explore and understand the molecular mechanisms involving miRNAs in regulating developmental and other gene expression for beneficial insects. However, examination of miRNAs associated with Hemiptera, especially predatory bugs, has been absent or scarce. This study represents the first comprehensive analysis of predatory bug A. chinensis encoded miRNAs through high throughput sequencing and predicts genes and biological processes regulated by the newly identified miRNAs through analyzing their differential expression in and across five nymphal instars. A total of 64 A. chinensis miRNAs, including 46 conserved miRNAs and 18 novel miRNAs, were identified by analysis of high throughput sequence reads mapped to the genome. A total of 2913 potential gene targets for these 64 miRNAs were predicted by comprehensive analyses utilizing miRanda, PITA, and RNAhybrid. Gene Ontology annotation of predicted target genes of A. chinensis suggested the key processes regulated by miRNAs involved biological processes, regulation of cellular processes, and transporter activity. Kyoto Encyclopedia of Genes and Genomes pathway predictions included the Toll and Imd signaling pathway, Valine, leucine and isoleucine degradation, Steroid biosynthesis, the AGE-RAGE signaling pathway in diabetic complications, and Alanine, aspartate and glutamate metabolism. This newly identified miRNAs through analyzing their differential expression, assessment of their predicted functions forms a foundation for further investigation of specific miRNAs.
Substances chimiques
MicroRNAs
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e21796Subventions
Organisme : the National Key Research and Development Program of China
ID : 2018YFD0201502
Organisme : the National Key Research and Development Program of China
ID : 2017YFD0200400
Organisme : Sino-America Biocontrol International Cooperation Program
ID : 59-0212-9-001-F
Informations de copyright
© 2021 Wiley Periodicals LLC.
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