Chromophore reduction plus reversible photobleaching: how the mKate2 "photoconversion" works.
Chromophore
Dithionite
FRET
Fluorescent protein
Photobleaching
Photoconversion
Journal
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
ISSN: 1474-9092
Titre abrégé: Photochem Photobiol Sci
Pays: England
ID NLM: 101124451
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
02
03
2021
accepted:
25
05
2021
pubmed:
5
6
2021
medline:
5
8
2021
entrez:
4
6
2021
Statut:
ppublish
Résumé
mKate red-to-green photoconversion is a non-canonical type of phototransformation in fluorescent proteins, with a poorly understood mechanism. We have hypothesized that the daughter mKate2 protein may also be photoconvertible, and that this phenomenon would be connected with mKate(2) chromophore photoreduction. Indeed, upon the intense irradiation of the protein sample supplemented by sodium dithionite, the accumulation of green as well as blue spectral forms is enhanced. The reaction was shown to be reversible upon the reductant's removal. However, an analysis of the fluorescence microscopy data, absorption spectra, kinetics and time-resolved fluorescence spectroscopy revealed that the short-wavelength spectral forms of mKate(2) exist before photoactivation, that their fractions increase light-independently after dithionite addition, and that the conversion is facilitated by the photobleaching of the red chromophore form.
Identifiants
pubmed: 34085171
doi: 10.1007/s43630-021-00060-8
pii: 10.1007/s43630-021-00060-8
doi:
Substances chimiques
Luminescent Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
791-803Subventions
Organisme : Russian Science Foundation (RSCF)
ID : 20-14-00255
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