Biosynthesis of Soyasapogenol B by Engineered Saccharomyces cerevisiae.
Metabolic engineering
Overexpression
Saccharomyces cerevisiae
Soyasapogenol B
β-amyrin
Journal
Applied biochemistry and biotechnology
ISSN: 1559-0291
Titre abrégé: Appl Biochem Biotechnol
Pays: United States
ID NLM: 8208561
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
received:
18
02
2021
accepted:
28
05
2021
pubmed:
8
6
2021
medline:
31
12
2021
entrez:
7
6
2021
Statut:
ppublish
Résumé
Soyasapogenol B is an oleanane-type pentacyclic triterpene that has various applications in food and healthcare and has a higher biological activity than soyasaponin. Saccharomyces cerevisiae is a potential platform for terpenoid production with mature genetic tools for metabolic pathway manipulation. In this study, we developed a biosynthesis method to produce soyasapogenol B. First, we expressed β-amyrin synthase derived from Glycyrrhiza glabra in S. cerevisiae to generate β-amyrin, as the precursor of soyasapogenol B. Several different types of promoters were then used to regulate the expression of key genes in the mevalonate pathway (MVA), and this subsequently increased the yield of β-amyrin to 17.6 mg/L, 25-fold more than that produced in the original strain L01 (0.68 mg/L). Then, using the β-amyrin-producing strain, we expressed soyasapogenol B synthases from Medicago truncatula (CYP93E2 and CYP72A61V2) and from G. glabra (CYP93E3 and CYP72A566). Soyasapogenol B yields were then optimized by using soyasapogenol B synthases and cytochrome P450 reductase from G. glabra. The most effective soyasapogenol B production strain was used for fermentation, and the yield of soyasapogenol B reached 2.9 mg/L in flask and 8.36 mg/L in a 5-L bioreactor with fed glucose and ethanol. This study demonstrated the heterologous synthesis of soyasapogenol B in S. cerevisiae using the combined expression of CYP93E3 and CYP72A566 in the synthesis pathway, which significantly increased the production of soyasapogenol B and provides a reference method for the biosynthesis of other triterpenes.
Identifiants
pubmed: 34097255
doi: 10.1007/s12010-021-03599-5
pii: 10.1007/s12010-021-03599-5
doi:
Substances chimiques
Saponins
0
oleanane
0
soyasapogenol B
1EZ10D7E2F
Oleanolic Acid
6SMK8R7TGJ
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3202-3213Subventions
Organisme : This work was financially supported by the Tianjin Natural Science Foundation (NO. 20JCQNJC00740) and the National Key Research and Development Program of China (No. 2019YFA0905100)
ID : (NO. 20JCQNJC00740); (No. 2019YFA0905100)
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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