Internal Fragments Generated from Different Top-Down Mass Spectrometry Fragmentation Methods Extend Protein Sequence Coverage.
CAD
UVPD
electron capture dissociation
electron ionization dissociation
internal fragments
top-down mass spectrometry
Journal
Journal of the American Society for Mass Spectrometry
ISSN: 1879-1123
Titre abrégé: J Am Soc Mass Spectrom
Pays: United States
ID NLM: 9010412
Informations de publication
Date de publication:
07 Jul 2021
07 Jul 2021
Historique:
pubmed:
9
6
2021
medline:
4
1
2022
entrez:
8
6
2021
Statut:
ppublish
Résumé
Top-down mass spectrometry (TD-MS) of intact proteins results in fragment ions that can be correlated to the protein primary sequence. Fragments generated can either be terminal fragments that contain the N- or C-terminus or internal fragments that contain neither termini. Traditionally in TD-MS experiments, the generation of internal fragments has been avoided because of ambiguity in assigning these fragments. Here, we demonstrate that in TD-MS experiments internal fragments can be formed and assigned in collision-based, electron-based, and photon-based fragmentation methods and are rich with sequence information, allowing for a greater extent of the primary protein sequence to be explained. For the three test proteins cytochrome
Identifiants
pubmed: 34101447
doi: 10.1021/jasms.1c00113
pmc: PMC9090460
mid: NIHMS1801024
doi:
Substances chimiques
Peptide Fragments
0
Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1752-1758Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM103533
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM103479
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR028893
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007185
Pays : United States
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