Probing the biogenesis pathway and dynamics of thylakoid membranes.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
09 06 2021
09 06 2021
Historique:
received:
05
08
2020
accepted:
11
05
2021
entrez:
10
6
2021
pubmed:
11
6
2021
medline:
23
6
2021
Statut:
epublish
Résumé
How thylakoid membranes are generated to form a metabolically active membrane network and how thylakoid membranes orchestrate the insertion and localization of protein complexes for efficient electron flux remain elusive. Here, we develop a method to modulate thylakoid biogenesis in the rod-shaped cyanobacterium Synechococcus elongatus PCC 7942 by modulating light intensity during cell growth, and probe the spatial-temporal stepwise biogenesis process of thylakoid membranes in cells. Our results reveal that the plasma membrane and regularly arranged concentric thylakoid layers have no physical connections. The newly synthesized thylakoid membrane fragments emerge between the plasma membrane and pre-existing thylakoids. Photosystem I monomers appear in the thylakoid membranes earlier than other mature photosystem assemblies, followed by generation of Photosystem I trimers and Photosystem II complexes. Redistribution of photosynthetic complexes during thylakoid biogenesis ensures establishment of the spatial organization of the functional thylakoid network. This study provides insights into the dynamic biogenesis process and maturation of the functional photosynthetic machinery.
Identifiants
pubmed: 34108457
doi: 10.1038/s41467-021-23680-1
pii: 10.1038/s41467-021-23680-1
pmc: PMC8190092
doi:
Substances chimiques
Bacterial Proteins
0
Photosynthetic Reaction Center Complex Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3475Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R003211/1
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : P50 GM082251
Pays : United States
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R003890/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R00370X/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S003339/1
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : R01 GM085043
Pays : United States
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R01390X/1
Pays : United Kingdom
Organisme : Medical Research Council
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M024202/1
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 206422/Z/17/Z
Pays : United Kingdom
Références
Photosynth Res. 1992 Apr;32(1):45-57
pubmed: 24408154
J Biol Chem. 2004 Nov 19;279(47):48620-9
pubmed: 15347679
Sci Rep. 2019 Jun 19;9(1):8725
pubmed: 31217458
Nat Plants. 2019 Apr;5(4):436-446
pubmed: 30962530
Plant Physiol. 2013 May;162(1):484-95
pubmed: 23463783
Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13443-8
pubmed: 11687660
Elife. 2015 Jan 13;4:
pubmed: 25584625
Front Microbiol. 2019 Feb 22;10:277
pubmed: 30853950
Nat Plants. 2020 Sep;6(9):1179-1191
pubmed: 32895528
Mol Plant. 2017 Nov 6;10(11):1434-1448
pubmed: 29017828
Ann Bot. 2010 Jul;106(1):1-16
pubmed: 20338950
Biochim Biophys Acta. 2016 Mar;1857(3):274-87
pubmed: 26592144
Plant Cell Physiol. 2016 Apr;57(4):878
pubmed: 27095830
Plant Physiol. 2016 Jun;171(2):1307-19
pubmed: 27208274
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11431-6
pubmed: 22733774
J Struct Biol. 2017 Feb;197(2):191-198
pubmed: 27313000
Plant Physiol. 2016 Nov;172(3):1451-1464
pubmed: 27621424
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):4243-8
pubmed: 11274448
Plant Physiol. 2016 May;171(1):530-41
pubmed: 26956667
Mol Biol Cell. 2009 Nov;20(21):4620-8
pubmed: 19776353
Plant Physiol. 2013 Oct;163(2):1037-46
pubmed: 23922268
Annu Rev Plant Biol. 2016 Apr 29;67:55-80
pubmed: 26735062
Biochim Biophys Acta. 2002 Sep 10;1555(1-3):204-9
pubmed: 12206916
J Struct Biol. 2017 Feb;197(2):102-113
pubmed: 27444392
Arch Microbiol. 2006 Jan;184(5):259-70
pubmed: 16320037
FEBS Lett. 1993 Dec 27;336(2):330-4
pubmed: 8262256
Mol Plant. 2017 Jan 9;10(1):62-72
pubmed: 27530366
Proc Natl Acad Sci U S A. 2020 Jul 21;117(29):17418-17428
pubmed: 32636267
Plant Cell. 2012 Feb;24(2):660-75
pubmed: 22319052
Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):4238-42
pubmed: 11274447
Plant Physiol. 1980 Dec;66(6):1144-9
pubmed: 16661593
Nat Plants. 2020 Jul;6(7):869-882
pubmed: 32665651
Plant Cell. 2004 Dec;16(12):3326-40
pubmed: 15548742
PLoS One. 2009;4(4):e5331
pubmed: 19390625
Biochim Biophys Acta. 2014 Apr;1837(4):503-11
pubmed: 24316145
Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19286-91
pubmed: 23129655
Biochim Biophys Acta. 2011 Aug;1807(8):935-44
pubmed: 21035426
Plant Physiol. 2019 Jan;179(1):184-194
pubmed: 30389782
J Biol Chem. 2008 Aug 15;283(33):22390-9
pubmed: 18550538
Philos Trans R Soc Lond B Biol Sci. 2012 Dec 19;367(1608):3444-54
pubmed: 23148271
EMBO J. 2007 Mar 7;26(5):1467-73
pubmed: 17304210
Mol Microbiol. 2015 May;96(3):448-62
pubmed: 25601560
Curr Biol. 2016 Nov 7;26(21):2862-2872
pubmed: 27720620
Plant Cell. 2019 Jul;31(7):1648-1664
pubmed: 31048338
J Theor Biol. 1972 Jul;36(1):105-17
pubmed: 5070894
Plant Physiol. 1993 Nov;103(3):835-843
pubmed: 12231982
Annu Rev Microbiol. 2020 Sep 8;74:633-654
pubmed: 32689916
Commun Biol. 2020 Oct 21;3(1):595
pubmed: 33087858
Biochim Biophys Acta. 2016 Mar;1857(3):256-65
pubmed: 26619924
J Proteomics. 2010 Mar 10;73(5):976-91
pubmed: 20060077
Arch Mikrobiol. 1971;78(1):25-41
pubmed: 4999393
Mol Plant. 2019 Sep 2;12(9):1176-1178
pubmed: 31376575
Plant J. 2007 Apr;50(2):265-77
pubmed: 17355436
FEMS Microbiol Lett. 2011 Feb;315(1):1-5
pubmed: 20831593
J Struct Biol. 2005 Oct;152(1):36-51
pubmed: 16182563
Plant Cell. 2001 Apr;13(4):793-806
pubmed: 11283337
EMBO J. 1996 May 1;15(9):2160-8
pubmed: 8641281
J Bacteriol. 2001 Jul;183(14):4251-8
pubmed: 11418566
J Biol Chem. 1998 Nov 6;273(45):29315-20
pubmed: 9792631
Plant Physiol. 2011 Apr;155(4):1656-66
pubmed: 21173021
Plant Physiol. 2006 Aug;141(4):1436-45
pubmed: 16798943
Plant Physiol. 2012 Jan;158(1):476-86
pubmed: 22086423
Nature. 2019 Feb;566(7744):411-414
pubmed: 30742075
Plant Cell. 2007 Nov;19(11):3640-54
pubmed: 18055604
Structure. 2017 Sep 5;25(9):1380-1390.e5
pubmed: 28803692
J Biol Chem. 2009 Jan 16;284(3):1813-9
pubmed: 19017636
FEBS J. 2005 Feb;272(4):892-902
pubmed: 15691324
Nat Methods. 2017 Apr;14(4):331-332
pubmed: 28250466
Mol Plant. 2017 Jan 9;10(1):143-154
pubmed: 27777125
Mol Plant. 2020 Jul 6;13(7):949-951
pubmed: 32416266
Nat Commun. 2020 Apr 24;11(1):1976
pubmed: 32332738
Nat Plants. 2021 Apr;7(4):524-538
pubmed: 33846594
Annu Rev Plant Biol. 2013;64:609-35
pubmed: 23451783
Biochim Biophys Acta. 2015 Sep;1847(9):838-48
pubmed: 25582571
Plant Physiol. 2012 Dec;160(4):2239-50
pubmed: 23037506
Nat Methods. 2017 Oct;14(10):983-985
pubmed: 28846087
Nat Commun. 2016 Aug 12;7:12471
pubmed: 27514992