Chromosomal Analysis of Ctenolucius hujeta Valenciennes, 1850 (Characiformes): A New Piece in the Chromosomal Evolution of the Ctenoluciidae.
Animals
Characiformes
/ classification
Chromosome Banding
Chromosomes
/ genetics
Cytogenetic Analysis
/ methods
Diploidy
Evolution, Molecular
Female
Genome
/ genetics
In Situ Hybridization, Fluorescence
/ methods
Karyotype
Karyotyping
/ methods
Male
RNA, Ribosomal, 18S
/ genetics
RNA, Ribosomal, 5S
/ genetics
Telomere
/ genetics
FISH
Fiber-FISH
Neotropical fish fauna
Pike characins
rDNA synteny
Journal
Cytogenetic and genome research
ISSN: 1424-859X
Titre abrégé: Cytogenet Genome Res
Pays: Switzerland
ID NLM: 101142708
Informations de publication
Date de publication:
2021
2021
Historique:
received:
25
08
2020
accepted:
25
02
2021
pubmed:
15
6
2021
medline:
3
8
2021
entrez:
14
6
2021
Statut:
ppublish
Résumé
Ctenoluciidae (Characiformes), a family of freshwater fishes, comprises 2 genera, Ctenolucius and Boulengerella, with 7 recognized species. Up to now, only species of the genus Boulengerella have been subjected to cytogenetic studies. Here, we investigated the karyotype and other cytogenetic features of pike characin, Ctenolucius hujeta, using conventional (Giemsa staining, C-banding, Ag-NOR staining) and molecular (rDNA, telomeric sequences, and fiber-FISH mapping) procedures. This species has a diploid chromosome number of 2n = 36, and a karyotype composed of 12m + 20sm + 4a and FN = 68, similar to that found in Boulengerella species. However, differences regarding the number and distribution of several chromosomal markers support a distinct generic status. Colocalization of the 18S and 5S rDNA genes is an exclusive characteristic of the C. hujeta genome, with an interspersed distribution in the chromosomal fiber, an unusual phenomenon among eukaryotes. Additionally, our results support the view that Ctenoluciidae and Lebiasinidae families are closely related.
Identifiants
pubmed: 34126615
pii: 000515456
doi: 10.1159/000515456
doi:
Substances chimiques
RNA, Ribosomal, 18S
0
RNA, Ribosomal, 5S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
195-202Informations de copyright
© 2021 S. Karger AG, Basel.