Correct dosage of X chromosome transcription is controlled by a nuclear pore component.
Acetylation
Animals
Dosage Compensation, Genetic
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ genetics
Genes, Insect
Genes, X-Linked
Histones
/ metabolism
Lysine
/ metabolism
Male
Nuclear Pore
/ genetics
RNA Transport
/ genetics
RNA, Messenger
/ genetics
TOR Serine-Threonine Kinases
/ metabolism
Transcription, Genetic
Up-Regulation
/ genetics
X Chromosome
/ genetics
MSL
Megator
Mtor
X chromosome
dosage compensation
nuclear pore complex
nucleoporin
transcription
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
15 06 2021
15 06 2021
Historique:
received:
23
07
2020
revised:
10
03
2021
accepted:
18
05
2021
entrez:
16
6
2021
pubmed:
17
6
2021
medline:
11
2
2022
Statut:
ppublish
Résumé
Dosage compensation in Drosophila melanogaster involves a 2-fold transcriptional upregulation of the male X chromosome, which relies on the X-chromosome-binding males-specific lethal (MSL) complex. However, how such 2-fold precision is accomplished remains unclear. Here, we show that a nuclear pore component, Mtor, is involved in setting the correct levels of transcription from the male X chromosome. Using larval tissues, we demonstrate that the depletion of Mtor results in selective upregulation at MSL targets of the male X, beyond the required 2-fold. Mtor and MSL components interact genetically, and depletion of Mtor can rescue the male lethality phenotype of MSL components. Using RNA fluorescence in situ hybridization (FISH) analysis and nascent transcript sequencing, we find that the effect of Mtor is not due to defects in mRNA export but occurs at the level of nascent transcription. These findings demonstrate a physiological role for Mtor in the process of dosage compensation, as a transcriptional attenuator of X chromosome gene expression.
Identifiants
pubmed: 34133927
pii: S2211-1247(21)00595-7
doi: 10.1016/j.celrep.2021.109236
pmc: PMC8224986
mid: NIHMS1715701
pii:
doi:
Substances chimiques
Drosophila Proteins
0
Histones
0
RNA, Messenger
0
TOR Serine-Threonine Kinases
EC 2.7.11.1
Lysine
K3Z4F929H6
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
109236Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM124143
Pays : United States
Organisme : NIGMS NIH HHS
ID : F31 GM133161
Pays : United States
Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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