Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
18 06 2021
18 06 2021
Historique:
received:
28
01
2021
accepted:
25
05
2021
entrez:
19
6
2021
pubmed:
20
6
2021
medline:
13
8
2021
Statut:
epublish
Résumé
PP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the malaria parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, consisting of three rounds of DNA synthesis, successive spindle formation with clustered kinetochores, and a meiotic stage during zygote to ookinete development following fertilization. It is unclear how PP1 is involved in these unusual processes. Using real-time live-cell and ultrastructural imaging, conditional gene knockdown, RNA-seq and proteomic approaches, we show that Plasmodium PP1 is implicated in both mitotic exit and, potentially, establishing cell polarity during zygote development in the mosquito midgut, suggesting that small molecule inhibitors of PP1 should be explored for blocking parasite transmission.
Identifiants
pubmed: 34145386
doi: 10.1038/s42003-021-02273-0
pii: 10.1038/s42003-021-02273-0
pmc: PMC8213788
doi:
Substances chimiques
Protozoan Proteins
0
Protein Phosphatase 1
EC 3.1.3.16
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
760Subventions
Organisme : Medical Research Council
ID : G0900109
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K011782/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/N017609/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : G0900278
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N023048/1
Pays : United Kingdom
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