Thymidylate kinase (TMK) of the photosynthetic, nitrogen-fixing cyanobacterium Nostoc sp. strain PCC7120: Biophysical, biochemical and physiological characterisation.
Cyanobacteria
Isothermal titration calorimetry
Luciferase-based TMK assay
Nostoc PCC7120
Photosystem II
Thymidylate kinase
Journal
Plant physiology and biochemistry : PPB
ISSN: 1873-2690
Titre abrégé: Plant Physiol Biochem
Pays: France
ID NLM: 9882449
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
06
05
2021
accepted:
10
06
2021
pubmed:
23
6
2021
medline:
8
9
2021
entrez:
22
6
2021
Statut:
ppublish
Résumé
Thymidylate kinase (TMK/TMPK) is an important enzyme in DNA biosynthesis and catalyses the conversion of dTMP to dTDP. Due to its therapeutic potential, the focus has been on characterizing the TMK proteins of pathogens and human origin, with very little information available on the TMK proteins of photosynthetic organisms and agriculturally important nitrogen-fixing organisms. In this work we report the characterisation of TMK in an evolutionarily ancient organism, cyanobacteria. The TMK protein of the photosynthetic, nitrogen-fixing cyanobacterium Nostoc PCC7120 (AnTMK) was found to have low conformational stability, which related to its low Tm of ~46 °C confirmed by Differential Scanning Fluorimetry (DSF) and Differential Scanning Calorimetry (DSC) techniques. The AnTMK protein exhibited substrate specificity for dTMP and ATP with K
Identifiants
pubmed: 34157604
pii: S0981-9428(21)00332-6
doi: 10.1016/j.plaphy.2021.06.019
pii:
doi:
Substances chimiques
Nucleoside-Phosphate Kinase
EC 2.7.4.4
dTMP kinase
EC 2.7.4.9
Nitrogen
N762921K75
Chlorophyll A
YF5Q9EJC8Y
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
416-426Informations de copyright
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