The impact of transcription factors Znf1, Sip4, Adr1, Tup1, and Hap4 on xylose alcoholic fermentation in the engineered yeast Saccharomyces cerevisiae.
Alcoholic fermentation
S. cerevisiae
Transcription factors
Xylose
Journal
Antonie van Leeuwenhoek
ISSN: 1572-9699
Titre abrégé: Antonie Van Leeuwenhoek
Pays: Netherlands
ID NLM: 0372625
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
09
02
2021
accepted:
18
06
2021
pubmed:
26
6
2021
medline:
25
8
2021
entrez:
25
6
2021
Statut:
ppublish
Résumé
Lignocellulosic biomass is an attractive sustainable platform for fuel ethanol production. Xylose is a second after glucose most abounded sugar in lignocellulosic hydrolysates. Effective conversion of xylose to ethanol is one of key prerequisite for the development of an efficient conversion of biomass to ethanol. Engineered Saccharomyces cerevisiae strains are able to xylose fermentation. However, the yield and productivities of xylose fermentation remains lower in comparison with glucose fermentation. In this work, we studied impact of transcription factors Znf1, Sip4, Adr1, Tup1, and Hap4 on xylose catabolism. We have isolated znf1Δ, adr1Δ, tup1Δ and hap4Δ mutants, and strains overexpressing SIP4, ADR1 and HAP4 genes on the background of xylose-fermenting strain of S. cerevisiae aiming to explore involvement of these transcription factors in regulation of xylose growth and fermentation. It was shown that hap4Δ reveal 1.8-fold increase of ethanol production from xylose as compared to that of parental strain. The hap4Δ mutant accumulates 10.38 g l
Identifiants
pubmed: 34170419
doi: 10.1007/s10482-021-01607-6
pii: 10.1007/s10482-021-01607-6
doi:
Substances chimiques
DNA-Binding Proteins
0
Nuclear Proteins
0
Repressor Proteins
0
Saccharomyces cerevisiae Proteins
0
TUP1 protein, S cerevisiae
0
Transcription Factors
0
Znf1 protein, S cerevisiae
0
Xylose
A1TA934AKO
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1373-1385Subventions
Organisme : Polish National Science Center
ID : UMO-2016/21/B/NZ1/00280 and DEC-2020/37/B/NZ1/02232
Organisme : National Academy of Sciences of Ukraine
ID : Grants 2-21 and 17-21
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
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