Survival of viable but nonculturable Cronobacter sakazakii in macrophages contributes to infections.

Animals Cronobacter sakazakii Endotoxins Infant Formula Macrophages Rats
Bacteremia C. sakazakii Macrophages VBNC Virulence

Journal

Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191

Informations de publication

Date de publication:
Sep 2021
Historique:
received: 15 02 2021
revised: 09 04 2021
accepted: 16 06 2021
pubmed: 26 6 2021
medline: 19 8 2021
entrez: 25 6 2021
Statut: ppublish

Résumé

Cronobacter sakazakii (C. sakazakii), a pathogen that exists in dry and low-moisture environments, such as powder infant formula (PIF), can enter a viable but nonculturable (VBNC) state under harsh conditions, which enables it to escape traditional detection methods and thus poses a potential public health risk. This study aimed at assessing the virulent nature of VBNC C. sakazakii. Our results showed that VBNC C. sakazakii induced intestinal inflammation in neonatal rats. However, the degree of inflammation was significantly lower than that of culturable bacteria due to decreasing endotoxin production, motility, adhesion, and invasion ability in the VBNC state. From the perspective of bacterial translocation, the numbers of C. sakazakii in the blood, liver, and spleen of rats treated with VBNC cells were in the same order of magnitude as those treated with its culturable counterpart and may lead to the same degree of bacteremia. According to the macrophage survival assays, the survival rate of VBNC C. sakazakii within macrophages was 4.7 times higher than that of culturable cells. Based on these findings, we hypothesize that VBNC C. sakazakii evaded the host immune defense system, penetrated the tissue barrier, and translocated to the bloodstream, liver, and spleen through macrophages. Thus, our study reveals that VBNC C. sakazakii could be a potential risk for infants' health.

Identifiants

DOI: 10.1016/j.micpath.2021.105064 PMID: 34171399
pubmed: 34171399
pii: S0882-4010(21)00336-3
doi: 10.1016/j.micpath.2021.105064
pii:
doi:

Substances chimiques

Endotoxins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

105064

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Aidi Zhou (A)

Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Wu Shan Road 483, Guangzhou 510642, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, PR China.

Li Wang (L)

Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Wu Shan Road 483, Guangzhou 510642, PR China. Electronic address: wangli_scau@scau.edu.cn.

Jingfeng Zhang (J)

Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Wu Shan Road 483, Guangzhou 510642, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, PR China.

Xiaoqing Yang (X)

Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Wu Shan Road 483, Guangzhou 510642, PR China.

Zhihua Ou (Z)

Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Wu Shan Road 483, Guangzhou 510642, PR China.

Lichao Zhao (L)

Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Wu Shan Road 483, Guangzhou 510642, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, PR China. Electronic address: zlc@scau.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Survival of viable but nonculturable...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Cronobacter › Cronobacter sakazakii › Survival of viable but nonculturable...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Toxines bactériennes › Endotoxines › Survival of viable but nonculturable...
questionsmedicales.fr › Aliments et boissons › Aliments › Aliments spécifiques › Aliment du nourrisson au cours de la première année › Préparation pour nourrissons › Survival of viable but nonculturable...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Macrophages › Survival of viable but nonculturable...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Survival of viable but nonculturable...