The plant NADPH oxidase RBOHD is required for microbiota homeostasis in leaves.
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
received:
10
01
2021
accepted:
25
05
2021
entrez:
1
7
2021
pubmed:
2
7
2021
medline:
21
9
2021
Statut:
ppublish
Résumé
The plant microbiota consists of a multitude of microorganisms that can affect plant health and fitness. However, it is currently unclear how the plant shapes its leaf microbiota and what role the plant immune system plays in this process. Here, we evaluated Arabidopsis thaliana mutants with defects in different parts of the immune system for an altered bacterial community assembly using a gnotobiotic system. While higher-order mutants in receptors that recognize microbial features and in defence hormone signalling showed substantial microbial community alterations, the absence of the plant NADPH oxidase RBOHD caused the most pronounced change in the composition of the leaf microbiota. The rbohD knockout resulted in an enrichment of specific bacteria. Among these, we identified Xanthomonas strains as opportunistic pathogens that colonized wild-type plants asymptomatically but caused disease in rbohD knockout plants. Strain dropout experiments revealed that the lack of RBOHD unlocks the pathogenicity of individual microbiota members driving dysbiosis in rbohD knockout plants. For full protection, healthy plants require both a functional immune system and a microbial community. Our results show that the NADPH oxidase RBOHD is essential for microbiota homeostasis and emphasizes the importance of the plant immune system in controlling the leaf microbiota.
Identifiants
pubmed: 34194036
doi: 10.1038/s41564-021-00929-5
pii: 10.1038/s41564-021-00929-5
pmc: PMC7612668
mid: EMS126426
doi:
Substances chimiques
Arabidopsis Proteins
0
respiratory burst oxidase homolog D, Arabidopsis
EC 1.6.-
NADPH Oxidases
EC 1.6.3.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
852-864Subventions
Organisme : European Research Council
ID : 668991
Pays : International
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