Redirected nuclear glutamate dehydrogenase supplies Tet3 with α-ketoglutarate in neurons.
Animals
Brain
/ metabolism
Cell Nucleus
/ enzymology
Citric Acid Cycle
Dioxygenases
/ genetics
Epigenomics
Gene Expression
Glutamate Dehydrogenase
/ genetics
Glutamic Acid
/ metabolism
HEK293 Cells
Humans
Ketoglutarate Dehydrogenase Complex
/ metabolism
Ketoglutaric Acids
/ metabolism
Metabolomics
Mice
Mice, Inbred C57BL
Mice, Knockout
Mitochondria
/ metabolism
Neuronal Plasticity
Neurons
/ metabolism
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
02 07 2021
02 07 2021
Historique:
received:
05
03
2021
accepted:
11
06
2021
entrez:
3
7
2021
pubmed:
4
7
2021
medline:
23
7
2021
Statut:
epublish
Résumé
Tet3 is the main α-ketoglutarate (αKG)-dependent dioxygenase in neurons that converts 5-methyl-dC into 5-hydroxymethyl-dC and further on to 5-formyl- and 5-carboxy-dC. Neurons possess high levels of 5-hydroxymethyl-dC that further increase during neural activity to establish transcriptional plasticity required for learning and memory functions. How αKG, which is mainly generated in mitochondria as an intermediate of the tricarboxylic acid cycle, is made available in the nucleus has remained an unresolved question in the connection between metabolism and epigenetics. We show that in neurons the mitochondrial enzyme glutamate dehydrogenase, which converts glutamate into αKG in an NAD
Identifiants
pubmed: 34215750
doi: 10.1038/s41467-021-24353-9
pii: 10.1038/s41467-021-24353-9
pmc: PMC8253819
doi:
Substances chimiques
Ketoglutaric Acids
0
Glutamic Acid
3KX376GY7L
TET3 protein, human
EC 1.-
Dioxygenases
EC 1.13.11.-
Tet3 protein, mouse
EC 1.13.11.-
Ketoglutarate Dehydrogenase Complex
EC 1.2.4.2
Glutamate Dehydrogenase
EC 1.4.1.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4100Références
Nucleic Acids Res. 2002 May 1;30(9):e36
pubmed: 11972351
J Org Chem. 2000 May 9;65(9):2813-5
pubmed: 10808462
Nat Methods. 2014 Mar;11(3):319-24
pubmed: 24487582
Nat Chem Biol. 2016 Jul;12(7):482-9
pubmed: 27159581
Methods. 2008 Jul;45(3):227-32
pubmed: 18620061
Cell. 2014 Jul 3;158(1):84-97
pubmed: 24995980
Neurochem Int. 2011 Sep;59(4):456-64
pubmed: 21108979
Nat Struct Mol Biol. 2007 Nov;14(11):1049-55
pubmed: 17984967
Nucleic Acids Res. 2020 Jan 8;48(D1):D440-D444
pubmed: 31691833
Neuron. 2013 Sep 18;79(6):1086-93
pubmed: 24050399
Nucleic Acids Res. 2016 Jan 4;44(D1):D447-56
pubmed: 26527722
Nat Neurosci. 2015 Jun;18(6):836-43
pubmed: 25915473
Science. 2009 May 15;324(5929):929-30
pubmed: 19372393
Nucleic Acids Res. 2010 Oct;38(19):e181
pubmed: 20685817
Biology (Basel). 2017 Feb 08;6(1):
pubmed: 28208702
Genes Cells. 2014 Jan;19(1):52-65
pubmed: 24304661
Nature. 2017 Jun 15;546(7658):381-386
pubmed: 28562591
Cell Metab. 2016 Oct 11;24(4):542-554
pubmed: 27641099
J Biol Chem. 2016 Feb 19;291(8):4256-65
pubmed: 26703470
Nat Neurosci. 2011 Aug 28;14(10):1345-51
pubmed: 21874013
Neurochem Res. 1986 May;11(5):733-44
pubmed: 3523273
Nat Protoc. 2019 Jan;14(1):283-312
pubmed: 30559375
Nucleic Acids Res. 1983 Mar 11;11(5):1475-89
pubmed: 6828386
Cell. 2017 Jan 12;168(1-2):210-223.e11
pubmed: 28086092
Cancer Cell. 2015 Feb 9;27(2):257-70
pubmed: 25670081
Sci Rep. 2017 May 19;7(1):2193
pubmed: 28526819
Science. 1993 Apr 9;260(5105):181-6
pubmed: 8097060
Biochem Cell Biol. 2009 Jun;87(3):505-16
pubmed: 19448744
Nature. 2010 Aug 26;466(7310):1129-33
pubmed: 20639862
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Trends Biochem Sci. 2016 Aug;41(8):712-730
pubmed: 27345518
J Cereb Blood Flow Metab. 2018 Oct;38(10):1754-1768
pubmed: 28621566
Mol Cell. 2014 Nov 6;56(3):414-424
pubmed: 25458842
Angew Chem Int Ed Engl. 2015 Oct 12;54(42):12511-4
pubmed: 26137924
Cell Chem Biol. 2016 Jan 21;23(1):74-85
pubmed: 26933737
Arch Immunol Ther Exp (Warsz). 2017 Feb;65(1):21-36
pubmed: 27326424
Chem Rev. 2015 Mar 25;115(6):2225-39
pubmed: 25675246
Trends Neurosci. 2016 Apr;39(4):264-275
pubmed: 26987258
Science. 2009 May 15;324(5929):930-5
pubmed: 19372391
Angew Chem Int Ed Engl. 2018 Apr 9;57(16):4296-4312
pubmed: 28941008
Nucleic Acids Res. 2014 Jul;42(13):8592-604
pubmed: 24948610
Mol Biol Cell. 2012 Sep;23(18):3677-93
pubmed: 22833565
Dev Cell. 2013 Feb 11;24(3):310-23
pubmed: 23352810
Anal Chim Acta. 2017 Aug 8;980:1-24
pubmed: 28622799
Nucleic Acids Res. 2001 May 1;29(9):e45
pubmed: 11328886
Science. 2009 Feb 20;323(5917):1074-7
pubmed: 19119186
Cell Metab. 2016 Sep 13;24(3):485-493
pubmed: 27476976
Science. 2018 May 11;360(6389):
pubmed: 29748257
Crit Rev Biochem Mol Biol. 2015;50(4):284-97
pubmed: 25837229
Science. 2017 Nov 17;358(6365):941-946
pubmed: 29025995
EMBO J. 2013 Mar 6;32(5):645-55
pubmed: 23353889
RNA Biol. 2017 Sep 2;14(9):1099-1107
pubmed: 28440690
Epigenomics. 2014;6(6):563-6
pubmed: 25531248
Development. 2012 Jun;139(11):1895-902
pubmed: 22569552
Proteomics. 2005 Jan;5(1):35-45
pubmed: 15602774
Mol Cell Proteomics. 2014 Sep;13(9):2513-26
pubmed: 24942700
Nat Chem Biol. 2018 Jan;14(1):72-78
pubmed: 29176672
Genes Brain Behav. 2011 Mar;10(2):137-48
pubmed: 20846178
J Biol Chem. 2015 Feb 20;290(8):4801-4812
pubmed: 25568311
Cell Mol Neurobiol. 2018 Apr;38(3):579-593
pubmed: 28623429
Nat Struct Mol Biol. 2012 Oct;19(10):1037-43
pubmed: 22961382
Science. 2011 Dec 23;334(6063):1669-75
pubmed: 22194569
Nature. 2015 Feb 19;518(7539):413-6
pubmed: 25487152
PLoS One. 2013 Jul 29;8(7):e70486
pubmed: 23922997
Cell Rep. 2015 Apr 14;11(2):283-94
pubmed: 25843715
PLoS One. 2013 May 14;8(5):e62755
pubmed: 23690950
J Inherit Metab Dis. 2013 Jul;36(4):595-612
pubmed: 23109059
PLoS One. 2010 Dec 23;5(12):e15367
pubmed: 21203455
Molecules. 2019 Apr 24;24(8):
pubmed: 31022909
J Neurosci. 1999 Jan 1;19(1):48-55
pubmed: 9870937
ACS Chem Neurosci. 2010 Jan 20;1(1):4-12
pubmed: 22778802
Am J Hum Genet. 2020 Feb 6;106(2):234-245
pubmed: 31928709
Diabetes. 2018 Oct;67(10):1949-1961
pubmed: 30002133