Transcriptome Dynamics of Epidermal Reprogramming during Direct Shoot Regeneration in Torenia fournieri.
Cytokinin •
Epidermal reprogramming •
Shoot apical meristem •
Shoot regeneration •
Torenia fournieri •
Transcriptome
Journal
Plant & cell physiology
ISSN: 1471-9053
Titre abrégé: Plant Cell Physiol
Pays: Japan
ID NLM: 9430925
Informations de publication
Date de publication:
10 Nov 2021
10 Nov 2021
Historique:
received:
17
02
2021
revised:
23
05
2021
accepted:
05
07
2021
pubmed:
6
7
2021
medline:
18
12
2021
entrez:
5
7
2021
Statut:
ppublish
Résumé
Shoot regeneration involves reprogramming of somatic cells and de novo organization of shoot apical meristems (SAMs). In the best-studied model system of shoot regeneration using Arabidopsis, regeneration is mediated by the auxin-responsive pluripotent callus formation from pericycle or pericycle-like tissues according to the lateral root development pathway. In contrast, shoot regeneration can be induced directly from fully differentiated epidermal cells of stem explants of Torenia fournieri (Torenia), without intervening the callus mass formation in culture with cytokinin; yet, its molecular mechanisms remain unaddressed. Here, we characterized this direct shoot regeneration by cytological observation and transcriptome analyses. The results showed that the gene expression profile rapidly changes upon culture to acquire a mixed signature of multiple organs/tissues, possibly associated with epidermal reprogramming. Comparison of transcriptomes between three different callus-inducing cultures (callus induction by auxin, callus induction by wounding and protoplast culture) of Arabidopsis and the Torenia stem culture identified genes upregulated in all the four culture systems as candidates of common factors of cell reprogramming. These initial changes proceeded independently of cytokinin, followed by cytokinin-dependent, transcriptional activations of nucleolar development and cell cycle. Later, SAM regulatory genes became highly expressed, leading to SAM organization in the foci of proliferating cells in the epidermal layer. Our findings revealed three distinct phases with different transcriptomic and regulatory features during direct shoot regeneration from the epidermis in Torenia, which provides a basis for further investigation of shoot regeneration in this unique culture system.
Identifiants
pubmed: 34223624
pii: 6314727
doi: 10.1093/pcp/pcab101
pmc: PMC8579340
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1335-1354Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP17K07437
Organisme : Japan Society for the Promotion of Science
ID : JP18H04830
Organisme : Japan Society for the Promotion of Science
ID : JP19J13924
Organisme : Japan Society for the Promotion of Science
ID : JP20H04881
Organisme : Exploratory Research for Advanced Technology
ID : JPMJER1004
Organisme : Japan Science Society
ID : No. 29-405
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.
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