Construction of Caffeine-Inducible Gene Switches in Mammalian Cells.
Caffeine
Inducible expression
Receptors
Synthetic biology
Transcription factors
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
6
7
2021
pubmed:
7
7
2021
medline:
12
8
2021
Statut:
ppublish
Résumé
Controlling gene expression in mammalian cells constitutes one of the core principles of mammalian synthetic biology. Especially for cell-based therapies, inducers of gene expression which demonstrate the highest degree of safety and patient adherence are needed. In this chapter, I describe methods to implement caffeine-controlled gene expression systems into mammalian cells. Using an array of different implementation strategies, from reconstituting transcription factors to activating endogenous signaling pathways, allows for a wide range of sensitivity and capacity of the resulting caffeine-responsive gene switches.
Identifiants
pubmed: 34228290
doi: 10.1007/978-1-0716-1441-9_10
doi:
Substances chimiques
Intracellular Signaling Peptides and Proteins
0
Transcription Factors
0
Caffeine
3G6A5W338E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
159-168Références
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