Biochemical Methods to Analyze the Subcellular Localization of NF-κB Proteins Using Cell Fractionation.
Centrifugation
Cytoplasmatic protein
NF-κB
Nuclear protein
Sucrose
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
8
7
2021
pubmed:
9
7
2021
medline:
13
1
2022
Statut:
ppublish
Résumé
Cell fractionation is a method used to study different cellular events like protein translocation and sequestration by disrupting cells and fractionating their contents, thus allowing an enrichment of the protein of interest. Using different concentrations of sucrose or detergent buffer formulations in combination with centrifugations, the cell fractions are separated based on their density and size.
Identifiants
pubmed: 34236630
doi: 10.1007/978-1-0716-1669-7_2
doi:
Substances chimiques
NF-kappa B
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19-25Références
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