Caffey disease is associated with distinct arginine to cysteine substitutions in the proα1(I) chain of type I procollagen.


Journal

Genetics in medicine : official journal of the American College of Medical Genetics
ISSN: 1530-0366
Titre abrégé: Genet Med
Pays: United States
ID NLM: 9815831

Informations de publication

Date de publication:
12 2021
Historique:
received: 21 07 2020
accepted: 30 06 2021
revised: 30 06 2021
pubmed: 18 7 2021
medline: 23 3 2022
entrez: 17 7 2021
Statut: ppublish

Résumé

Infantile Caffey disease is a rare disorder characterized by acute inflammation with subperiosteal new bone formation, associated with fever, pain, and swelling of the overlying soft tissue. Symptoms arise within the first weeks after birth and spontaneously resolve before the age of two years. Many, but not all, affected individuals carry the heterozygous pathogenic COL1A1 variant (c.3040C>T, p.(Arg1014Cys)). We sequenced COL1A1 in 28 families with a suspicion of Caffey disease and performed ultrastructural, immunocytochemical, and biochemical collagen studies on patient skin biopsies. We identified the p.(Arg1014Cys) variant in 23 families and discovered a novel heterozygous pathogenic COL1A1 variant (c.2752C>T, p.(Arg918Cys)) in five. Both arginine to cysteine substitutions are located in the triple helical domain of the proα1(I) procollagen chain. Dermal fibroblasts (one patient with p.(Arg1014Cys) and one with p.(Arg918Cys)) produced molecules with disulfide-linked proα1(I) chains, which were secreted only with p.(Arg1014Cys). No intracellular accumulation of type I procollagen was detected. The dermis revealed mild ultrastructural abnormalities in collagen fibril diameter and packing. The discovery of this novel pathogenic variant expands the limited spectrum of arginine to cysteine substitutions in type I procollagen. Furthermore, it confirms allelic heterogeneity in Caffey disease and impacts its molecular confirmation.

Identifiants

pubmed: 34272483
doi: 10.1038/s41436-021-01274-y
pii: S1098-3600(21)05445-9
doi:

Substances chimiques

COL1A1 protein, human 0
Collagen Type I 0
Collagen Type I, alpha 1 Chain 0
Procollagen 0
Procollagen Type I 0
Arginine 94ZLA3W45F
Cysteine K848JZ4886

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2378-2385

Informations de copyright

© 2021. The Author(s), under exclusive licence to the American College of Medical Genetics and Genomics.

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Auteurs

Tibbe Dhooge (T)

Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, Ghent University Hospital, Ghent, Belgium.

Delfien Syx (D)

Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, Ghent University Hospital, Ghent, Belgium.

Trinh Hermanns-Lê (T)

Department of Dermatopathology, University Hospital of Sart-Tilman, Liège University, Liège, Belgium.

Ingrid Hausser (I)

Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.

Geert Mortier (G)

Department of Medical Genetics, Antwerp University Hospital and University of Antwerp, Antwerp, Belgium.

Jonathan Zonana (J)

Department of Molecular and Medical Genetics, Oregon Health and Sciences University, Portland, OR, USA.

Sofie Symoens (S)

Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, Ghent University Hospital, Ghent, Belgium.

Peter H Byers (PH)

Department of Pathology and Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA, USA.

Fransiska Malfait (F)

Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, Ghent University Hospital, Ghent, Belgium. Fransiska.Malfait@UGent.be.

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