NANOG gene suppression and replacement of let-7 modulate the stemness, invasion, and apoptosis in breast cancer.
Antigens, CD
/ genetics
Apoptosis
/ genetics
Breast Neoplasms
/ genetics
Cadherins
/ genetics
Cell Line, Tumor
Cell Movement
/ genetics
Female
Gene Expression Regulation, Neoplastic
HMGA2 Protein
/ genetics
Humans
MicroRNAs
/ genetics
Nanog Homeobox Protein
/ genetics
Neoplastic Stem Cells
/ pathology
Octamer Transcription Factor-3
/ genetics
RNA, Small Interfering
RNA-Binding Proteins
/ genetics
Spheroids, Cellular
/ pathology
Transfection
Vimentin
/ genetics
Apoptosis
Breast cancer
Let-7
Migration
NANOG
Stemness
Journal
Gene
ISSN: 1879-0038
Titre abrégé: Gene
Pays: Netherlands
ID NLM: 7706761
Informations de publication
Date de publication:
30 Oct 2021
30 Oct 2021
Historique:
received:
14
03
2021
revised:
06
06
2021
accepted:
13
07
2021
pubmed:
19
7
2021
medline:
17
8
2021
entrez:
18
7
2021
Statut:
ppublish
Résumé
In the treatment of breast cancer (BC), as an important type of cancer in women, the specific cells, called cancer stem cells (CSCs), are the reason of failure and metastasis. So, targeting CSCs can be used as a novel strategy in cancer therapy in addition to common therapeutic strategies. According to the importance of CSCs, we tried to find a correlation between stemness and metastatic characteristics of BC cells, to address whether CSCs are a potential target for cancer therapy. Here, we evaluated the NANOG inhibition by siRNA and the increase of Let-7a levels by miRNA mimic in breast cancer cells and the effects of these changes on biologic aspects like cell apoptosis, stemness and invasion. Our results showed that the inhibition of NANOG combined with Let-7a restoration contributed to significant decrease in malignant phenotypes and stemness feature of BC cells. In conclusion, these findings showed that the combination of Let-7a miRNA mimic and Nanog siRNA could be exploited as a new treatment strategy to improve the cancer therapy outcome.
Identifiants
pubmed: 34274471
pii: S0378-1119(21)00439-X
doi: 10.1016/j.gene.2021.145844
pii:
doi:
Substances chimiques
Antigens, CD
0
CDH1 protein, human
0
Cadherins
0
HMGA2 Protein
0
HMGA2 protein, human
0
Lin28A protein, human
0
MicroRNAs
0
NANOG protein, human
0
Nanog Homeobox Protein
0
Octamer Transcription Factor-3
0
POU5F1 protein, human
0
RNA, Small Interfering
0
RNA-Binding Proteins
0
VIM protein, human
0
Vimentin
0
mirnlet7 microRNA, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
145844Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.