Rapid protein delivery to living cells for biomolecular investigation.
In-cell EPR
Protein delivery
Protein dynamic
Structural biology
Thermal vesiculation
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
17 09 2021
17 09 2021
Historique:
received:
01
07
2021
accepted:
04
07
2021
pubmed:
19
7
2021
medline:
18
11
2021
entrez:
18
7
2021
Statut:
ppublish
Résumé
The lack of a simple, fast and efficient method for protein delivery is limiting the widespread application of in-cell experiments, which are crucial for understanding the cellular function. We present here an innovative strategy to deliver proteins into both prokaryotic and eukaryotic cells, exploiting thermal vesiculation. This method allows to internalize substantial amounts of proteins, with different molecular weight and conformation, without compromising the structural properties and cell viability. Characterizing proteins in a physiological environment is essential as the environment can dramatically affect the conformation and dynamics of biomolecules as shown by in-cell EPR spectra vs those acquired in buffer solution. Considering its versatility, this method opens the possibility to scientists to study proteins directly in living cells through a wide range of techniques.
Identifiants
pubmed: 34274850
pii: S0006-291X(21)01029-9
doi: 10.1016/j.bbrc.2021.07.006
pii:
doi:
Substances chimiques
Proteins
0
enhanced green fluorescent protein
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
82-88Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare no competing interests.