Interstrand Aminoacyl Transfer in a tRNA Acceptor Stem-Overhang Mimic.
Journal
Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056
Informations de publication
Date de publication:
04 08 2021
04 08 2021
Historique:
pubmed:
21
7
2021
medline:
10
2
2022
entrez:
20
7
2021
Statut:
ppublish
Résumé
Protein-catalyzed aminoacylation of the 3'-overhang of tRNA by an aminoacyl-adenylate could not have taken place prior to the advent of genetically coded peptide synthesis, and yet the latter process has an absolute requirement for aminoacyl-tRNA. There must therefore have been an earlier nonprotein-catalyzed means of generating aminoacyl-tRNA. Here, we demonstrate efficient interstrand aminoacyl transfer from an aminoacyl phosphate mixed anhydride at the 5'-terminus of a tRNA acceptor stem mimic to the 2',3'-diol terminus of a short 3'-overhang. With certain five-base 3'-overhangs, the transfer of an alanyl residue is highly stereoselective with the l-enantiomer being favored to the extent of ∼10:1 over the d-enantiomer and is much more efficient than the transfer of a glycyl residue.
Identifiants
pubmed: 34283595
doi: 10.1021/jacs.1c05746
pmc: PMC8397310
doi:
Substances chimiques
RNA, Transfer
9014-25-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11836-11842Subventions
Organisme : Medical Research Council
ID : MC_UP_A024_1009
Pays : United Kingdom
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