Development and successful real-world use of a transfer DNA technique to identify species involved in shark bite incidents.
marine predator
mucus
shark attack
shark bite
species barcoding
swab kit
trace evidence
traumatogenic wild animal
Journal
Journal of forensic sciences
ISSN: 1556-4029
Titre abrégé: J Forensic Sci
Pays: United States
ID NLM: 0375370
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
revised:
30
06
2021
received:
29
11
2020
accepted:
07
07
2021
pubmed:
22
7
2021
medline:
19
11
2021
entrez:
21
7
2021
Statut:
ppublish
Résumé
Identifying the species involved in shark bite incidents is an ongoing challenge but is important to mitigate risk. We developed a sampling protocol to identify shark species from DNA transferred to inanimate objects during bite incidents. To develop and refine the technique, we swabbed shark bite impressions on surfboards and wetsuit neoprene collected under semicontrolled conditions. Methods were tested experimentally and then successfully used to identify the species involved in a real-world shark bite incident. Thirty-two of 33 bite impressions yielded sufficient DNA sequences for species identification, producing barcodes from five test species, including dusky, Galapagos, bull, tiger, and white shark. The latter three species collectively account for a majority of shark bites worldwide. Our method successfully identified the species (Galeocerdo cuvier) responsible for a fatal shark bite on December 8th, 2020 on the island of Maui, from swab samples collected from the victim's surfboard 49 h after the bite incident. Our experimental results demonstrate that shark species can be accurately identified from transfer DNA recovered from bite impressions on surfboards and wetsuit neoprene. The successful use of our method in the real-world incident shows great potential for the practicality of this tool. We recommend DNA swabbing as a routine part of the forensic analysis of shark bites to help identify the species involved in human-shark interactions.
Identifiants
pubmed: 34286850
doi: 10.1111/1556-4029.14808
doi:
Substances chimiques
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2438-2443Subventions
Organisme : Hawaii Department of Land and Natural Resources
Organisme : Save Our Seas Foundation
Informations de copyright
© 2021 American Academy of Forensic Sciences.
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