Transcriptome analysis identifies putative genes involved in triterpenoid biosynthesis in Platycodon grandiflorus.
Differentially expressed genes
Full-length transcriptome
RNA-Seq
SMRT sequencing
Triterpenoid saponin biosynthesis
Journal
Planta
ISSN: 1432-2048
Titre abrégé: Planta
Pays: Germany
ID NLM: 1250576
Informations de publication
Date de publication:
21 Jul 2021
21 Jul 2021
Historique:
received:
17
03
2021
accepted:
30
06
2021
entrez:
22
7
2021
pubmed:
23
7
2021
medline:
24
7
2021
Statut:
epublish
Résumé
Comprehensive transcriptome analysis of different Platycodon grandiflorus tissues discovered genes related to triterpenoid saponin biosynthesis. Platycodon grandiflorus (Jacq.) A. DC. (P. grandiflorus), a traditional Chinese medicine, contains considerable triterpenoid saponins with broad pharmacological activities. Triterpenoid saponins are the major components of P. grandiflorus. Here, single-molecule real-time and next-generation sequencing technologies were combined to comprehensively analyse the transcriptome and identify genes involved in triterpenoid saponin biosynthesis in P. grandiflorus. We quantified four saponins in P. grandiflorus and found that their total content was highest in the roots and lowest in the stems and leaves. A total of 173,354 non-redundant transcripts were generated from the PacBio platform, and three full-length transcripts of β-amyrin synthase, the key synthase of β-amyrin, were identified. A total of 132,610 clean reads obtained from the DNBSEQ platform were utilised to explore key genes related to the triterpenoid saponin biosynthetic pathway in P. grandiflorus, and 96 differentially expressed genes were selected as candidates. The expression levels of these genes were verified by quantitative real-time PCR. Our reliable transcriptome data provide valuable information on the related biosynthesis pathway and may provide insights into the molecular mechanisms of triterpenoid saponin biosynthesis in P. grandiflorus.
Identifiants
pubmed: 34291354
doi: 10.1007/s00425-021-03677-2
pii: 10.1007/s00425-021-03677-2
doi:
Substances chimiques
Saponins
0
Triterpenes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
34Subventions
Organisme : Innovative Research Group Project of the National Natural Science Foundation of China
ID : 82073957
Organisme : Innovative Research Group Project of the National Natural Science Foundation of China
ID : 81773853
Organisme : University Natural Science Research Project of Anhui Province
ID : 1808085QH290
Organisme : The CAMS Innovation Fund for Medical Sciences
ID : 2019-I2M-5-065
Organisme : The Key Project at the Central Government Level: The Ability Establishment of Sustainable Use for ValuableChinese Medicine Resources
ID : 2060302
Organisme : The Special Fundfor Guiding Local Science and Technology Development
ID : YDZX20183400004233
Organisme : The Major scientific and technological projects in Anhui Province
ID : 18030801128
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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