Methylation of Promoters of Apoptosis-Related Genes in Blood Lymphocytes of Workers Exposed to Occupational External Irradiation.

Aged Apoptosis / genetics Apoptosis Regulatory Proteins / genetics Apoptotic Protease-Activating Factor 1 / genetics BH3 Interacting Domain Death Agonist Protein / genetics Chromosome Aberrations / classification DNA Methylation Epigenesis, Genetic Gamma Rays / adverse effects Humans Lymphocytes / metabolism Male Middle Aged Occupational Exposure / adverse effects Promoter Regions, Genetic Radiation Exposure / adverse effects Radiometry Repressor Proteins / genetics Siberia Tumor Suppressor Proteins / genetics bcl-2-Associated X Protein / genetics bcl-Associated Death Protein / genetics

DNA methylation a promoter apoptosis chromosomal aberrations ionizing radiation

Journal

Bulletin of experimental biology and medicine

ISSN: 1573-8221

Titre abrégé: Bull Exp Biol Med

Pays: United States

ID NLM: 0372557

Informations de publication

Date de publication:
Jul 2021

Historique:

received: 13 11 2020

pubmed: 24 7 2021

medline: 8 1 2022

entrez: 23 7 2021

Statut: ppublish

Résumé

We studied the effect of technogenic radiation on the degree of promoter methylation in genes involved in apoptosis in blood lymphocytes of workers exposed to long-term γ-radiation during their professional activities. Blood samples for the analysis were obtained from 11 conventionally healthy men aged from 54 to 71 years (mean 66 years), workers of the Siberian Group of Chemical Enterprises working experience from 27 to 40 years (mean 30 years); the external exposure dose was 175.88 mSv (158.20-207.81 mSv). In all examined subjects, the degree of methylation of the promoters of apoptosis-related genes ranged from 0.22 to 50.00%. A correlation was found between the degree of methylation of BCLAF1 promoters (p=0.035) with the age of workers, BAX promoters (p=0.0289) with high content of aberrant cells, and APAF1 promoters (p=0.0152) with increased number of dicentric chromosomes. A relationship was found between the dose of external irradiation and the degree of methylation of gene promoters of BAD (p=0.0388), BID (р=0.0426), and HRK (р=0.0101) genes.

Identifiants

DOI: 10.1007/s10517-021-05227-y PMID: 34297287

pubmed: 34297287

doi: 10.1007/s10517-021-05227-y

pii: 10.1007/s10517-021-05227-y

doi:

Substances chimiques

APAF1 protein, human 0

Apoptosis Regulatory Proteins 0

Apoptotic Protease-Activating Factor 1 0

BAD protein, human 0

BAX protein, human 0

BCLAF1 protein, human 0

BH3 Interacting Domain Death Agonist Protein 0

BID protein, human 0

HRK protein, human 0

Repressor Proteins 0

Tumor Suppressor Proteins 0

bcl-2-Associated X Protein 0

bcl-Associated Death Protein 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

357-361

Informations de copyright

Références

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Methylation of Promoters of Apoptosis-Related Genes in Blood Lymphocytes of Workers Exposed to Occupational External Irradiation.

Journal

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Résumé

Identifiants

Substances chimiques

Types de publication

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Sous-ensembles de citation

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Références

Auteurs

D S Isubakova (DS)

O S Tsymbal (OS)

E V Bronikovskaya (EV)

N V Litviakov (NV)

I V Milto (IV)

R М Takhauov (RМ)

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