High-throughput sequencing of Fasciola spp. shows co-infection and intermediate forms in Balochistan, but only Fasciola gigantica in the Punjab province of Pakistan.
Animals
Buffaloes
Cattle
Cattle Diseases
/ epidemiology
Coinfection
/ epidemiology
DNA, Helminth
/ analysis
DNA, Mitochondrial
/ analysis
DNA, Ribosomal
/ analysis
Fasciola
/ isolation & purification
Fascioliasis
/ epidemiology
Goat Diseases
/ epidemiology
Goats
High-Throughput Nucleotide Sequencing
/ veterinary
Pakistan
/ epidemiology
Prevalence
Sheep
Sheep Diseases
/ epidemiology
Sheep, Domestic
Species Specificity
Fasciola gigantica
Fasciola hepatica
Fasciolosis
mtDNA ND-1
rDNA ITS-2
Journal
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
ISSN: 1567-7257
Titre abrégé: Infect Genet Evol
Pays: Netherlands
ID NLM: 101084138
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
01
06
2021
revised:
16
07
2021
accepted:
19
07
2021
pubmed:
25
7
2021
medline:
18
1
2022
entrez:
24
7
2021
Statut:
ppublish
Résumé
Fasciola gigantica and Fasciola hepatica are digenetic trematodes causing fasciolosis in ruminants. The host and geographical distribution of both Fasciola species are influenced by environmental and climatic conditions favouring survival and development of free-living stages and intermediate hosts, and livestock management practices. The aim of the present study was to describe the host distribution of the two Fasciola species in buffalo, cattle, goats, and sheep in the Balochistan and Punjab provinces of Pakistan. 359 flukes were collected from a total of 32 livers from the four livestock species. Deep amplicon sequencing of the internal transcribed spacer region 2 of ribosomal DNA (rDNA ITS-2) and mitochondrial nicotinamide adenine dinucleotide dehydrogenase 1 (mtDNA ND-1) loci confirmed co-infection of F. hepatica and F. gigantica in Balochistan and single species F. gigantica infection in Punjab. In Balochistan, co-infections and hybrids of both Fasciola species were identified in cattle, with more F. hepatica detected than F. gigantica. However, F. hepatica was the only species identified in goats, and F. gigantica was the only species identified in buffalo. In Punjab, all flukes were confirmed as F. gigantica in each of the four livestock species. Overall, the results indicate differences in the host and geographical distribution of F. gigantica and F. hepatica, and provide useful knowledge for the development of control strategies for livestock and humans.
Identifiants
pubmed: 34302994
pii: S1567-1348(21)00310-5
doi: 10.1016/j.meegid.2021.105012
pii:
doi:
Substances chimiques
DNA, Helminth
0
DNA, Mitochondrial
0
DNA, Ribosomal
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105012Subventions
Organisme : Biotechnology and Biological Sciences Research Council
Pays : United Kingdom
Informations de copyright
Copyright © 2021. Published by Elsevier B.V.