Autocrine regulation of wound healing by ATP release and P2Y
Extracellular ATP
Keratinocyte
Purinergic receptor
Skin
Wound closure
Journal
Life sciences
ISSN: 1879-0631
Titre abrégé: Life Sci
Pays: Netherlands
ID NLM: 0375521
Informations de publication
Date de publication:
15 Oct 2021
15 Oct 2021
Historique:
received:
22
02
2021
revised:
05
07
2021
accepted:
17
07
2021
pubmed:
28
7
2021
medline:
14
9
2021
entrez:
27
7
2021
Statut:
ppublish
Résumé
Application of exogenous nucleotides can modulate wound healing via the activation of purinergic receptors. However, evidence for the release of endogenous nucleotides and the subsequent activation of purinergic receptors in this process has not been well defined. Therefore, the current study aimed to investigate wound-mediated nucleotide release and autocrine purinergic signalling during HaCaT keratinocyte wound closure following scratch injury. An in vitro scratch wound apparatus was employed to study wound healing over 24-h in the presence of modulators of ATP release, P2 receptors and pathways downstream of P2 receptor activation. Adenosine 5'-triphosphate (ATP) was released from scratched cells. The ectonucleotidase apyrase and pharmacological inhibition of the nucleotide release hemichannel, pannexin-1, decreased wound closure over time. The non-selective P2Y receptor antagonist suramin and the selective P2Y These data describe a novel autocrine signalling mechanism in which wound-mediated release of endogenous ATP in response to mechanical scratching of HaCaT cells activates P2Y
Identifiants
pubmed: 34314735
pii: S0024-3205(21)00837-7
doi: 10.1016/j.lfs.2021.119850
pii:
doi:
Substances chimiques
P2RY2 protein, human
0
Purinergic P2Y Receptor Antagonists
0
Receptors, Purinergic P2Y2
0
Suramin
6032D45BEM
Adenosine Triphosphate
8L70Q75FXE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
119850Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.