Development of a background signal suppression probe for 8-oxoguanine DNA glycosylase detection.

DNA Glycosylases / metabolism DNA Repair Guanine / analogs & derivatives
8-oxoguanine DNA glycosylase Background signal suppression Base excision repair Cancer Lambda exonuclease

Journal

Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534

Informations de publication

Date de publication:
29 Aug 2021
Historique:
received: 17 01 2021
revised: 18 05 2021
accepted: 03 06 2021
entrez: 31 7 2021
pubmed: 1 8 2021
medline: 4 8 2021
Statut: ppublish

Résumé

8-oxoguanine DNA glycosylase (OGG), which plays a crucial role in base excision repair (BER), is an important biomarker. The existing highly sensitive fluorescent methods always need complicated amplification design. The method with high sensitivity and simple design at the same time is urgently needed. Here, we developed a highly sensitive detection method for OGG detection with lambda exonuclease and the background signal suppression probe. Through probe structure design, the steric hindrance and competitive binding effects successfully suppressed the background signal. We achieved sensitive detection of OGG with a simple design, and the limit of detection was 5.0 × 10

Identifiants

DOI: 10.1016/j.aca.2021.338741 PMID: 34330449
pubmed: 34330449
pii: S0003-2670(21)00567-5
doi: 10.1016/j.aca.2021.338741
pii:
doi:

Substances chimiques

8-hydroxyguanine 5614-64-2
Guanine 5Z93L87A1R
DNA Glycosylases EC 3.2.2.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

338741

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Bocheng Tu (B)

School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Zishan Feng (Z)

School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Haitao Wang (H)

State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, China.

Wei Zhang (W)

Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China; Department of Obstetrics and Gynaecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Weicong Ye (W)

School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Hongbo Wang (H)

Department of Obstetrics and Gynaecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Xianjin Xiao (X)

Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Wenbo Zhao (W)

State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, China. Electronic address: zhaowb1987@126.com.

Tongbo Wu (T)

School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. Electronic address: wutongbo@hust.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › N-Glycosyl hydrolases › DNA Glycosylases › Development of a background signal suppression...
questionsmedicales.fr › Phénomènes génétiques › Réparation de l'ADN › Development of a background signal suppression...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Purines › Purinones › Hypoxanthines › Guanine › Development of a background signal suppression...