Development of a protein assay with copper chelator chromeazurol B, based on the biuret reaction.
Automation analyzer
Biuret reaction
Chelate
Chromeazurol B
Colorimetric detection
Protein assay
Journal
Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535
Informations de publication
Date de publication:
01 10 2021
01 10 2021
Historique:
received:
26
05
2021
revised:
20
07
2021
accepted:
28
07
2021
pubmed:
4
8
2021
medline:
29
12
2021
entrez:
3
8
2021
Statut:
ppublish
Résumé
This study aimed to provide a novel and highly sensitive protein assay based on the biuret reaction and using chromeazurol B, a metal chelate compound. The method consists of two reagents and an automated analyzer. First, a complex of copper and protein (biuret reaction) is formed. Second, a chelating reagent containing chromeazurol B forms a three-dimensional complex of protein, copper, and chromeazurol B at neutral pH, resulting in highly sensitive coloration. The intra-assay (n = 20) variation for the three levels was 3.54 % or lower at each concentration. Each response with α, β-, and γ-globulin was 103.8 % and 104.3 %, respectively, against albumin. The molar absorption coefficient (ε) of the present method was 2.5 × 10
Identifiants
pubmed: 34343480
pii: S0003-2697(21)00221-9
doi: 10.1016/j.ab.2021.114320
pii:
doi:
Substances chimiques
Benzoates
0
Chelating Agents
0
Globulins
0
Hemoglobins
0
Organometallic Compounds
0
alpha 1-Antitrypsin
0
beta 2-Microglobulin
0
chromazurol B
1796-92-5
Copper
789U1901C5
Serum Albumin, Human
ZIF514RVZR
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
114320Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.