The intervening domain is required for DNA-binding and functional identity of plant MADS transcription factors.
AGAMOUS Protein, Arabidopsis
/ chemistry
Arabidopsis
/ genetics
Arabidopsis Proteins
/ genetics
DNA-Binding Proteins
/ chemistry
Flowers
Gene Expression Regulation, Plant
Homeodomain Proteins
/ chemistry
MADS Domain Proteins
/ metabolism
Phenotype
Protein Interaction Domains and Motifs
Transcription Factors
/ chemistry
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
06 08 2021
06 08 2021
Historique:
received:
31
05
2021
accepted:
14
07
2021
entrez:
7
8
2021
pubmed:
8
8
2021
medline:
24
8
2021
Statut:
epublish
Résumé
The MADS transcription factors (TF) are an ancient eukaryotic protein family. In plants, the family is divided into two main lineages. Here, we demonstrate that DNA binding in both lineages absolutely requires a short amino acid sequence C-terminal to the MADS domain (M domain) called the Intervening domain (I domain) that was previously defined only in type II lineage MADS. Structural elucidation of the MI domains from the floral regulator, SEPALLATA3 (SEP3), shows a conserved fold with the I domain acting to stabilise the M domain. Using the floral organ identity MADS TFs, SEP3, APETALA1 (AP1) and AGAMOUS (AG), domain swapping demonstrate that the I domain alters genome-wide DNA-binding specificity and dimerisation specificity. Introducing AG carrying the I domain of AP1 in the Arabidopsis ap1 mutant resulted in strong complementation and restoration of first and second whorl organs. Taken together, these data demonstrate that the I domain acts as an integral part of the DNA-binding domain and significantly contributes to the functional identity of the MADS TF.
Identifiants
pubmed: 34362909
doi: 10.1038/s41467-021-24978-w
pii: 10.1038/s41467-021-24978-w
pmc: PMC8346517
doi:
Substances chimiques
AGAMOUS Protein, Arabidopsis
0
AP1 protein, Arabidopsis
0
Arabidopsis Proteins
0
DNA-Binding Proteins
0
Homeodomain Proteins
0
MADS Domain Proteins
0
SEP3 protein, Arabidopsis
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4760Informations de copyright
© 2021. The Author(s).
Références
Nature. 2001 Jan 25;409(6819):469-71
pubmed: 11206529
Proc Natl Acad Sci U S A. 2000 May 9;97(10):5328-33
pubmed: 10805792
Plant Physiol. 2009 Apr;149(4):1713-23
pubmed: 19211705
Genes Dev. 1991 Dec;5(12A):2327-41
pubmed: 1748287
PLoS Comput Biol. 2013;9(9):e1003214
pubmed: 24039567
Nucleic Acids Res. 2018 Jun 1;46(10):4966-4977
pubmed: 29562355
Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4063-70
pubmed: 8633017
Nucleic Acids Res. 2014;42(17):10927-42
pubmed: 25183521
J Synchrotron Radiat. 2010 Sep;17(5):700-7
pubmed: 20724792
Mol Biol Evol. 2016 Jun;33(6):1486-501
pubmed: 26908583
PLoS Biol. 2009 Apr 21;7(4):e1000090
pubmed: 19385720
Dev Cell. 2009 May;16(5):711-22
pubmed: 19460347
Acta Biochim Pol. 2001;48(2):351-8
pubmed: 11732606
Plant Physiol. 2008 Sep;148(1):259-68
pubmed: 18599653
Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13407-12
pubmed: 14597714
FEBS Lett. 2004 Mar 12;561(1-3):127-31
pubmed: 15013763
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67
pubmed: 21460454
Genome Res. 2018 Jun;28(6):891-900
pubmed: 29654070
Bioinformatics. 2016 Jul 15;32(14):2089-95
pubmed: 27153568
Plant J. 1998 Dec;16(6):735-43
pubmed: 10069079
Plant Cell. 2013 Jul;25(7):2482-503
pubmed: 23821642
Nucleic Acids Res. 1992 Jul 11;20(13):3297-303
pubmed: 1630900
EMBO J. 1990 Mar;9(3):605-13
pubmed: 1968830
Plant Physiol. 2010 Sep;154(1):287-300
pubmed: 20631316
Plant J. 1993 Aug;4(2):385-98
pubmed: 8106084
Plant J. 2015 Jul;83(2):344-58
pubmed: 25990192
Methods Mol Biol. 2017;1629:67-82
pubmed: 28623580
Gene. 2005 Mar 14;347(2):183-98
pubmed: 15777618
Nucleic Acids Res. 2009 Jan;37(1):144-57
pubmed: 19033361
Nature. 1995 Aug 10;376(6540):490-8
pubmed: 7637780
BMC Bioinformatics. 2018 Dec 20;19(1):536
pubmed: 30572828
Mol Biol Cell. 1997 Jul;8(7):1243-59
pubmed: 9243505
EMBO J. 1999 Oct 1;18(19):5370-9
pubmed: 10508169
Plant Cell. 2017 Aug;29(8):1822-1835
pubmed: 28733422
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):133-44
pubmed: 20124693
Nucleic Acids Res. 1993 Oct 11;21(20):4769-76
pubmed: 7901838
Nucleic Acids Res. 2003 Aug 1;31(15):4401-9
pubmed: 12888499
Proc Natl Acad Sci U S A. 1996 May 14;93(10):4793-8
pubmed: 8643482
Plant Physiol. 2003 Oct;133(2):462-9
pubmed: 14555774
Plant Cell. 1996 May;8(5):831-45
pubmed: 8672883
Nucleic Acids Res. 2020 Sep 25;48(17):9637-9648
pubmed: 32890394
Cell. 1988 Dec 23;55(6):989-1003
pubmed: 3203386
J Mol Biol. 2007 Sep 21;372(3):774-97
pubmed: 17681537
Mol Biol Evol. 2012 Jan;29(1):293-302
pubmed: 21813465
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Genome Biol. 2008;9(9):R137
pubmed: 18798982
Genome Res. 2004 Jun;14(6):1188-90
pubmed: 15173120
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Plant Cell. 2014 Sep;26(9):3603-15
pubmed: 25228343
Plant Cell. 2008 Aug;20(8):2088-101
pubmed: 18713950
Genome Biol. 2009 Feb 25;10(2):R24
pubmed: 19243611
Plant Cell. 2013 Jul;25(7):2560-72
pubmed: 23847151
Bioinformatics. 2015 Sep 1;31(17):2761-9
pubmed: 25957351
Plant J. 2001 May;26(4):385-94
pubmed: 11439126
Acta Crystallogr D Biol Crystallogr. 2007 Jan;63(Pt 1):32-41
pubmed: 17164524
Plant Cell. 2020 Nov;32(11):3408-3424
pubmed: 32873631
Genome Biol. 2010;11(6):214
pubmed: 20587009
J Mol Biol. 1988 Dec 5;204(3):593-606
pubmed: 3066908
Plant Cell. 2011 Mar;23(3):865-72
pubmed: 21378131
Plant Physiol. 2007 Jan;143(1):447-60
pubmed: 17071640
Nucleic Acids Res. 1996 Aug 15;24(16):3134-41
pubmed: 8774892
Plant Cell. 2005 May;17(5):1424-33
pubmed: 15805477
Proc Natl Acad Sci U S A. 2012 Jan 31;109(5):1560-5
pubmed: 22238427
Mol Plant. 2019 Jun 3;12(6):822-832
pubmed: 30336329
Plant Cell. 2007 Aug;19(8):2544-56
pubmed: 17693535
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Plant Cell. 1990 Aug;2(8):741-53
pubmed: 1983792
J Biol Chem. 2002 Jul 19;277(29):26429-35
pubmed: 11971906
J Mol Evol. 2003 May;56(5):573-86
pubmed: 12698294
Development. 2012 Sep;139(17):3081-98
pubmed: 22872082
Nature. 1990 Jul 5;346(6279):35-9
pubmed: 1973265
Nucleic Acids Res. 2009 Jul;37(Web Server issue):W202-8
pubmed: 19458158
Mol Phylogenet Evol. 2003 Dec;29(3):464-89
pubmed: 14615187