Nuclear export of plant pararetrovirus mRNAs involves the TREX complex, two viral proteins and the highly structured 5' leader region.
5' Untranslated Regions
Active Transport, Cell Nucleus
Arabidopsis
/ virology
Arabidopsis Proteins
/ metabolism
Capsid Proteins
/ metabolism
Caulimovirus
/ genetics
Cell Nucleus
/ metabolism
Plant Diseases
/ virology
RNA, Messenger
/ metabolism
RNA, Viral
/ chemistry
RNA-Directed DNA Polymerase
/ metabolism
Viral Proteins
/ metabolism
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
07 09 2021
07 09 2021
Historique:
accepted:
22
07
2021
revised:
09
07
2021
received:
01
08
2020
pubmed:
10
8
2021
medline:
11
9
2021
entrez:
9
8
2021
Statut:
ppublish
Résumé
In eukaryotes, the major nuclear export pathway for mature mRNAs uses the dimeric receptor TAP/p15, which is recruited to mRNAs via the multisubunit TREX complex, comprising the THO core and different export adaptors. Viruses that replicate in the nucleus adopt different strategies to hijack cellular export factors and achieve cytoplasmic translation of their mRNAs. No export receptors are known in plants, but Arabidopsis TREX resembles the mammalian complex, with a conserved hexameric THO core associated with ALY and UIEF proteins, as well as UAP56 and MOS11. The latter protein is an orthologue of mammalian CIP29. The nuclear export mechanism for viral mRNAs has not been described in plants. To understand this process, we investigated the export of mRNAs of the pararetrovirus CaMV in Arabidopsis and demonstrated that it is inhibited in plants deficient in ALY, MOS11 and/or TEX1. Deficiency for these factors renders plants partially resistant to CaMV infection. Two CaMV proteins, the coat protein P4 and reverse transcriptase P5, are important for nuclear export. P4 and P5 interact and co-localise in the nucleus with the cellular export factor MOS11. The highly structured 5' leader region of 35S RNAs was identified as an export enhancing element that interacts with ALY1, ALY3 and MOS11 in vitro.
Identifiants
pubmed: 34370034
pii: 6346659
doi: 10.1093/nar/gkab653
pmc: PMC8421220
doi:
Substances chimiques
5' Untranslated Regions
0
Arabidopsis Proteins
0
Capsid Proteins
0
RNA, Messenger
0
RNA, Viral
0
Viral Proteins
0
RNA-Directed DNA Polymerase
EC 2.7.7.49
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8900-8922Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
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