Immobilization of papain: A review.

Carica / enzymology Enzyme Stability Enzymes / chemistry Enzymes, Immobilized / chemistry Papain / chemistry
Enzyme immobilization Enzyme stabilization Protein hydrolysis

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
01 Oct 2021
Historique:
received: 02 07 2021
revised: 22 07 2021
accepted: 03 08 2021
pubmed: 11 8 2021
medline: 15 12 2021
entrez: 10 8 2021
Statut: ppublish

Résumé

Papain is a cysteine protease from papaya, with many applications due to its broad specificity. This paper reviews for first time the immobilization of papain on different supports (organic, inorganic or hybrid supports) presenting some of the features of the utilized immobilization strategies (e.g., epoxide, glutaraldehyde, genipin, glyoxyl for covalent immobilization). Special focus is placed on the preparation of magnetic biocatalysts, which will permit the simple recovery of the biocatalyst even if the medium is a suspension. Problems specific to the immobilization of proteases (e.g., steric problems when hydrolyzing large proteins) are also defined. The benefits of a proper immobilization (enzyme stabilization, widening of the operation window) are discussed, together with some artifacts that may suggest an enzyme stabilization that may be unrelated to enzyme rigidification.

Identifiants

DOI: 10.1016/j.ijbiomac.2021.08.016 PMID: 34375660
pubmed: 34375660
pii: S0141-8130(21)01675-5
doi: 10.1016/j.ijbiomac.2021.08.016
pii:
doi:

Substances chimiques

Enzymes 0
Enzymes, Immobilized 0
Papain EC 3.4.22.2

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

94-113

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Auteurs

Veymar G Tacias-Pascacio (VG)

Facultad de Ciencias de la Nutrición y Alimentos, Universidad de Ciencias y Artes de Chiapas, Lib. Norte Pte. 1150, 29039 Tuxtla Gutiérrez, Chiapas, Mexico; Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana Km. 1080, 29050 Tuxtla Gutiérrez, Chiapas, Mexico.

Roberto Morellon-Sterling (R)

Departamento de Biocatálisis. ICP-CSIC./Marie Curie 2, Campus UAM-CSIC Cantoblanco, 28049 Madrid. Spain; Student of Departamento de Biología Molecular, Universidad Autónoma de Madrid, Darwin 2, Campus UAM-CSIC, Cantoblanco, 28049 Madrid. Spain.

Daniel Castañeda-Valbuena (D)

Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana Km. 1080, 29050 Tuxtla Gutiérrez, Chiapas, Mexico.

Ángel Berenguer-Murcia (Á)

Departamento de Química Inorgánica e Instituto Universitario de Materiales, Universidad de Alicante, Alicante, Spain.

Majid Rasool Kamli (MR)

Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddad 21589, Saudi Arabia; Center of excellence in Bionanoscience Research, King Abdulaziz University, Jeddad 21589, Saudi Arabia.

Olga Tavano (O)

Faculty of Nutrition, Alfenas Federal Univ., 700 Gabriel Monteiro da Silva St, Alfenas, MG 37130-000, Brazil.

Roberto Fernandez-Lafuente (R)

Departamento de Biocatálisis. ICP-CSIC./Marie Curie 2, Campus UAM-CSIC Cantoblanco, 28049 Madrid. Spain; Center of Excellence in Bionanoscience Research, External advisory board, King Abdulaziz University, Jeddah 21589, Saudi Arabia. Electronic address: rfl@icp.csic.es.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Caricaceae › Carica › Immobilization of papain: A review.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité protéique › Stabilité enzymatique › Immobilization of papain: A review.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Immobilization of papain: A review.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines immobilisées › Enzymes immobilisées › Immobilization of papain: A review.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Endopeptidases › Cysteine endopeptidases › Papaïne › Immobilization of papain: A review.