Class A scavenger receptor-1/2 facilitates the uptake of bovine milk exosomes in murine bone marrow-derived macrophages and C57BL/6J mice.
Animals
Cattle
Clodronic Acid
/ pharmacology
Cytochalasin D
/ pharmacology
Endocytosis
/ drug effects
Exosomes
/ chemistry
Female
Fluorescent Dyes
/ chemistry
Gene Expression
Liver
/ drug effects
Lung
/ drug effects
Macrophages
/ cytology
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Milk
/ chemistry
Phagocytosis
/ drug effects
Protein Isoforms
/ deficiency
Scavenger Receptors, Class A
/ deficiency
Spleen
/ drug effects
Staining and Labeling
/ methods
bone marrow-derived macrophages
bovine
class A scavenger receptors
milk exosomes
phagocytosis
Journal
American journal of physiology. Cell physiology
ISSN: 1522-1563
Titre abrégé: Am J Physiol Cell Physiol
Pays: United States
ID NLM: 100901225
Informations de publication
Date de publication:
01 09 2021
01 09 2021
Historique:
pubmed:
12
8
2021
medline:
22
9
2021
entrez:
11
8
2021
Statut:
ppublish
Résumé
Bovine milk exosomes (BMEs) are being explored in drug delivery despite their rapid elimination by macrophages. We aimed at identifying the BME transporter in murine bone marrow-derived macrophages (BMDMs). Fluorophore-labeled BMEs were used in transport studies in BMDMs from C57BL/6J and class A scavenger receptor type 1/2 (CASR-1/2) knockout mice and tissue accumulation in macrophage-depleted C57BL/6J mice. Parametric and nonparametric statistics tests for pairwise and multiple comparisons were used. Chemical inhibitors of phagocytosis by cytochalasin D led to a 69 ± 18% decrease in BME uptake compared with controls (
Identifiants
pubmed: 34378992
doi: 10.1152/ajpcell.00222.2021
pmc: PMC8461812
doi:
Substances chimiques
Fluorescent Dyes
0
Msr1 protein, mouse
0
Protein Isoforms
0
Scavenger Receptors, Class A
0
Clodronic Acid
0813BZ6866
Cytochalasin D
22144-77-0
Banques de données
figshare
['10.6084/m9.figshare.14763363.v5']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
C607-C614Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM104320
Pays : United States
Organisme : HHS | NIH | National Institute of General Medical Sciences (NIGMS)
ID : P20GM104320
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