The Construction of a Genetically Encoded, Phage-Displayed Cyclic-Peptide Library.
Cyclic peptides
HDAC8
Nε-acryloyl-lysine
Phage display
Proximity-driven cyclization
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
13
8
2021
pubmed:
14
8
2021
medline:
12
1
2022
Statut:
ppublish
Résumé
Due to the great potentials of cyclic peptides as therapeutic agents, several phage-displayed peptide libraries in which cyclization is achieved by the covalent linkage of cysteines have been previously demonstrated to identify cyclic-peptide ligands for therapeutic targets. While problems remain in these cysteine conjugation strategies, we have invented a phage display technique in which its displayed peptides are cyclized through a proximity-driven Michael addition reaction between a cysteine and an amber-codon-encoded N
Identifiants
pubmed: 34386961
doi: 10.1007/978-1-0716-1617-8_17
pmc: PMC8491649
mid: NIHMS1741732
doi:
Substances chimiques
Ligands
0
Peptide Library
0
Peptides
0
Peptides, Cyclic
0
Cysteine
K848JZ4886
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
219-230Subventions
Organisme : NCI NIH HHS
ID : R01 CA161158
Pays : United States
Informations de copyright
© 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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