Tomotherapy Applied Total Lymphoid Irradiation and Allogeneic Hematopoietic Cell Transplantation Generates Mixed Chimerism in the Rhesus Macaque Model.
Journal
Radiation research
ISSN: 1938-5404
Titre abrégé: Radiat Res
Pays: United States
ID NLM: 0401245
Informations de publication
Date de publication:
01 12 2021
01 12 2021
Historique:
received:
04
11
2020
accepted:
27
07
2021
pubmed:
14
8
2021
medline:
31
12
2021
entrez:
13
8
2021
Statut:
ppublish
Résumé
Development of a new methodology to induce immunological chimerism after allogeneic hematopoietic cell (HC) transplantation in a rhesus macaque model is described. The chimeric state was achieved using a non-myeloablative, helical tomotherapy-based total lymphoid irradiation (TomoTLI) conditioning regimen followed by donor HC infusions between 1-haplotype matched donor/recipient pairs. The technique was tested as a feasibility study in an experimental group of seven rhesus macaques that received the novel TomoTLI tolerance protocol and HC allo-transplants. Two tomotherapy protocols were compared: TomoTLI (n = 5) and TomoTLI/total-body irradiation (TBI) (n = 2). Five of seven animals developed mixed chimerism. Three of five animals given the TomoTLI protocol generated transient mixed chimerism with no graft-versus-host disease (GVHD) with survival of 33, 152 and >180 days. However, the inclusion of belatacept in addition to a single fraction of TBI resulted in total chimerism and fatal GVHD in both animals, indicating an unacceptable conditioning regimen.
Identifiants
pubmed: 34388816
pii: 469433
doi: 10.1667/RADE-20-00246.1
pmc: PMC8826536
mid: NIHMS1769144
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
623-632Subventions
Organisme : NIH HHS
ID : P40 OD028116
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI125231
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI102456
Pays : United States
Organisme : NIAID NIH HHS
ID : U24 AI126683
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
©2021 by Radiation Research Society. All rights of reproduction in any form reserved.
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