Enhanced endogenous amino acids and energy metabolism level for cAMP biosynthesis by Arthrobacter sp. CCTCC 2013431 with citrate as cosubstrate.
Amino asids
Citrate
Cyclic adenosine monophosphate
Energy metabolism
Oxidative stress
Journal
Biotechnology letters
ISSN: 1573-6776
Titre abrégé: Biotechnol Lett
Pays: Netherlands
ID NLM: 8008051
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
received:
05
05
2021
accepted:
05
08
2021
pubmed:
16
8
2021
medline:
21
12
2021
entrez:
15
8
2021
Statut:
ppublish
Résumé
In our previous study, citrate was used as auxiliary energy substance for improving cAMP fermentation performance, however, the regulation mechanism of citrate on improved cAMP contents was not clear. To elucidate the regulation mechanism, cAMP fermentations with/without citrate addition were conducted in a 7 L fermentor using Arthrobacter sp. CCTCC 2013431 and assays on key enzymes activities, energy metabolism level, amino acids contents and peroxidation level were performed. With 3 g/L-broth sodium citrate added, cAMP concentration and conversion yield from glucose reached 4.34 g/L and 0.076 g/g which were improved by 30.7% and 29.8%, respectively, when compared with those of control. Citrate changed carbon flux distribution among different routes and more carbon flux was directed into pentose phosphate pathway beneficial to cAMP synthesis. Meanwhile, energy metabolism together with precursor amino acids levels were improved significantly owing to strengthened metabolic intensity of tricarboxylate cycle by exogenous citrate utilization which provided energy and substance basis for cAMP production. Moreover, higher glutamate synthesis and oxidative stress caused by citrate addition consumed excessive NADPH derived from pentose phosphate pathway by which feedback suppression for pentose phosphate pathway was relieved efficiently. Citrate promoted cAMP fermentation production by Arthrobacter sp. CCTCC 2013431 due to enhanced precursor amino acids, energy metabolism level and relieved feedback suppression for pentose phosphate pathway.
Identifiants
pubmed: 34392452
doi: 10.1007/s10529-021-03170-6
pii: 10.1007/s10529-021-03170-6
doi:
Substances chimiques
Amino Acids
0
Culture Media
0
Citric Acid
2968PHW8QP
Cyclic AMP
E0399OZS9N
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1989-1999Subventions
Organisme : Science and Technology Department of Henan Province
ID : 192102210193
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature B.V.
Références
CaoY ME, Shi ZP (2013) A novel metabolic model incorporating directed signal flow diagram with enzymatic activities data for evaluating glutamate yield in glutamate fermentation. Biochem Eng J 77:136–146
doi: 10.1016/j.bej.2013.05.015
Chen H, Cao X, Zhu NQ et al (2020) A stepwise control strategy for glutathione synthesis in Saccharomyces cerevisiae based on oxidative stress and energy metabolism. World J Microb Biot 36(8):177–182
doi: 10.1007/s11274-020-02895-2
Chen XC, Song H, Fang T et al (2010a) Enhanced cyclic adenosine monophosphate production by Arthrobacter A302 through rational redistribution of metabolic flux. Bioresource Techn 101:3159–3163
doi: 10.1016/j.biortech.2009.12.081
Chen Y, Li SY, Xiong J et al (2010b) The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5’-monophosphate by Saccharomyces cerevisiae. Appl Microbiol Biotechnol 86:75–81
doi: 10.1007/s00253-009-2287-y
Chen Y, Liu Q, Chen XC et al (2015) Redirecting metabolic flux in Saccharomyces cerevisiae through regulation of cofactors in UMP production. J Ind Microbiol Biotechnol 42:577–583
doi: 10.1007/s10295-014-1536-y
Comasio A, Harth H, Weckx S et al (2019) The addition of citrate stimulates the production of acetoin and diacetyl by a citrate-positive Lactobacillus crustorum strain during wheat sourdough fermentation. Int J Food Microbiol 289:88–105
doi: 10.1016/j.ijfoodmicro.2018.08.030
Gu Y, Tan H, Yuan LN et al (2021) Physiological mechanisms for enhanced cyclic adenosine monophosphate biosynthesis by sodium fluoride in Arthrobacter sp. Biotechnol Bull 37(5):108–116
Kang TS, Korber DR, Tanaka T (2013) Contributions of citrate in redox potential maintenance and ATP production: metabolic pathways and their regulation in Lactobacillus panis PM1. Appl Microbiol Biotechnol 97:8693–8703
doi: 10.1007/s00253-013-5108-2
Lelle M, Otte M, Thon S et al (2019) Chemical synthesis and biological activity of novel brominated 7-deazaadenosine-3’, 5’-cyclic monophosphate derivatives. Bioorgan Med Chem 27(8):1704–1713
doi: 10.1016/j.bmc.2019.03.024
Li S, Ji J, Hu S et al (2020) Enhancement of ε-poly-L-lysine production in Streptomyces griseofuscus by addition of exogenous astaxanthin. Bioproc Biosyst Eng 43(10):1813–1821
doi: 10.1007/s00449-020-02372-y
Li ZG, Chen BF, Fang ZB et al (2018) A novel fermentation process for cyclic adenosine monophosphate production based on citrate coupling hypoxanthine addition in pulses. Food Ferment Ind 44(11):154–158
Li ZG, Gu Y, Chen BF et al (2021) Physiological mechanism for enhanced cyclic adenosine monophosphate biosynthesis by sodium nitrate in Arthrobacter sp. CCTCC M2013431. Sci Technol Food Ind 42(1):115–120
Liu Q, Wu C, Huang S et al (2018) Decreased hyperpolarization-activated cyclic nucleotide-gated channels are involved in bladder dysfunction associated with spinal cord injury. Int J Mol Med 41(5):2609–2618
pubmed: 29436607
pmcid: 5846662
Niu HQ, Wang JZ, Zhuang W et al (2018) Comparative transcriptomic and proteomic analysis of Arthrobacter sp CGMCC 3584 responding to dissolved oxygen for cAMP production. Sci Rep. https://doi.org/10.1038/s41598-017-18889-4
doi: 10.1038/s41598-017-18889-4
pubmed: 30504917
pmcid: 6269485
Niu HQ, Sun XZ, Song J et al (2020) Knockout of pde gene in Arthrobacter sp. CGMCC 3584 and transcriptomic analysis of its effects on cAMP production. Bioproc Biosyst Eng 43(5):839–850
doi: 10.1007/s00449-019-02280-w
Sholokh A, Klussmann E (2021) Local cyclic adenosine monophosphate signalling cascades-roles and targets in chronic kidney disease. Acta Physiol. https://doi.org/10.1111/apha.13641
doi: 10.1111/apha.13641
Wang A, Tian W, Cheng L et al (2020) Enhanced ε-Poly-L-Lysine production by the synergistic effect of ε-Poly-L-Lysine synthetase overexpression and citrate in Streptomyces albulus. Front Bioeng Biotech. https://doi.org/10.3389/fbioe.2020.00288
doi: 10.3389/fbioe.2020.00288
Wang YL, Wang DH, Wei GY et al (2013) Improved co-production of S-adenosylmethionine and glutathione using citrate as an auxiliary energy substrate. Bioresource Techn 131:28–32
doi: 10.1016/j.biortech.2012.10.168
Xia J, Xu ZX, Xu H et al (2014) The regulatory effect of citric acid on the co-production of poly (ε-lysine) and poly (L-diaminopropionic acid) in Streptomyces albulus PD-1. Bioprocess Biosyst Eng 37:2095–2103
doi: 10.1007/s00449-014-1187-4
Zeng X, Chen XS, Gao Y et al (2015) Continuously high reactive oxygen species generation decreased the specific ε-poly-L-lysine formation rate in fed-batch fermentation using glucose and glycerol as a mixed carbon source. Process Biochem 50(12):1993–2003
doi: 10.1016/j.procbio.2015.09.012
Zeng X, Chen XS, Ren XD et al (2016) Improved ε-poly-L-lysine productivity partly resulting from rapid cell growth in cultures using a glucose-glycerol mixed carbon source. Eng Life Sci 16(5):1–10
doi: 10.1002/elsc.201500090
Zhou J, Liu L, Chen J (2010) Improved ATP supply enhances acid tolerance of Candida glabrata during pyruvic acid production. J Appl Microbiol 110:44–53
doi: 10.1111/j.1365-2672.2010.04865.x
Zhu Q, Zhuang W, Niu H et al (2018) Affinity induced immobilization of adenylate cyclase from the crude cell lysate for ATP conversion. Colloid Surface B 164:155–164
doi: 10.1016/j.colsurfb.2018.01.033